Mineralogical and exposure determinants of pulmonary fibrosis among Quebec chrysotile miners and millers.

摘要

Objectives: Lung fibre content was determined for 86 former chrysotile miners and millers in two Quebec mining regions: Thetford mines (TM) and the Asbestos region (AR). Methods: Fibres were assessed using transmission electron microscopy (TEM) and energy dispersive X-ray spectrometry (EDS). Asbestos body (AB) concentrations were assessed by microscopy of tissue digests. Corresponding histological lung tissue sections were quantitatively graded for the severity of interstitial fibrosis on a 12-point scale. Fibrosis score and its associations with (1) fibre concentrations and fibre dimensions within three fibre length intervals (less than 5 mum, 5-10 mum, and over 10 mum), and (2) several exposure variables were evaluated using correlation coefficients and regression techniques. Results: Concentration of short (<5 mum) tremolite fibres was the best predictor of fibrosis grade in both mining groups (r=0.44, P<0.01 and r=0.39, P<0.01 for TM and AR, respectively). Chrysotile fibre concentration showed a lower correlation with the fibrosis grade for subjects from TM only. Long (>10 mum) amosite fibre concentration showed a linear relationship with the fibrosis score in miners and millers from AR. Exposure variables, including smoking, had no predictive value for fibrosis grade. Within fibre length categories, fibre dimension was not related to the fibrosis score. Conclusion: Lung fibre concentration as measured by TEM/EDS, especially that of short (<5 mum) tremolite fibres, is a better predictor of fibrosis grade in these two groups of chrysotile miners than either the concentration of ABs or the duration of exposure. Due to the limitation of our counting method, almost all fibres longer than 10 mum observed in this study were shorter than 14 mum. Thus, if length plays a role in fibrogenesis, it may be related to fibres of greater length than those covered in this study.