A novel solid-phase nucleic acid extraction kit for high-throughput sample preparation

摘要

A diverse line of nucleic acid extraction applications with a nucleic acid extraction kit streamline this essential component of nucleic acid testing and interface directly with amplification. The Xtra Amp~(TM) kit (Xtrana, Inc., Broomfield, CO) provides a rapid method by which DNA or RNA may be extracted from a variety of biological samples and subsequently amplified by standard polymerase chain reaction (PCR) procedures in the same tube. The proprietary Xtra Bind~(TM) solid-phase nucleic acid binding material (Xtrana) has been incorporated into the kit. This kit has applications for genomic DNA extraction from whole blood (Figure 1), blood stains, huffy coat, buccal swabs, tissue culture cells, tissues, mouse/rat tails, yeast, as well as chromosomal or plasmid DNA extraction from E. coli, and total RNA extraction from tissue culture cells (Figure 2). Other applications currently in development include RNA extraction from blood and tissue, and DNA and RNA extraction from plant cells and viruses.With the Xtra Amp system, the nucleic acid is irreversibly bound to the solid-phase matrix and has shown very good stability, providing storage for extended time periods for ensuring analyses. DNA extracted with the kit can be stored dry at room temperature for later use or reuse. RNA can be extracted at room temperature, allowing high-throughput users to automate the extraction process. In addition, the extracted RNA can be stored for several weeks at 4 deg C, and indefinitely at -20 deg C. Additionalapplications include direct interface with PCR or alternative amplification methods, real-time amplification/detection (Figure 1), storage or archiving of bound nucleic acid, repeat gene analysis or multiplex genetic loci detection, and rapid automationof nucleic acid extraction.