A Comparative Analysis of Ultraviolet Light vs High-Heat Sterilization in a Cell Culture C0_2 incubator

摘要

In 2001, SANYO Electric Co., Ltd. (Osaka, Japan) introduced a cell culture COz incubator that employs an isolated narrow-bandwidth ultraviolet light to destroy airborne contaminants in the incubator chamber, as well as waterbome organisms in the humidity water reservoir. Comparative testing commissioned by SANYO in 2006 and performed by a certified independent testing laboratory (see Table. I) confirms that the SANYO ultraviolet light sterilization process is as effective against bacteria, yeasts, and molds as high-heat sterilization at sustained temperatures ranging from +90 deg C to +140 deg C offered in competitive products. Additionally, the model MCO-18AIC-UV CO_2 incubator (SANYO) (Figure I) isolates the UV light emission from cell cultures during normal operation to permit sterilization of the internal atmo-sphere following routine door openings without damaging cell cultures, a process that cannot be replicated with a heat sterilization technique.