Identification of lactic acid bacteria isolated from salted vegetables by DNA-DNA hybridization

摘要

This study was to establish a method using DNA-DNA hybridization to identify species of lactic acid bacteria isolated from various types of salted vegetables. Enough chromosomal DNA could be obtained from lactic acid bacteria by lysing with N-acetylmuramidase in combination with lysozome in order to carry out DNA-DNA hybridization. A total of 35 strains of lactic acid bacteria isolated from salted vegetables were of the Lactobacillus, Lactococcus, Leuconostoc and Pediococcus species, and these bacteria were identified by DNA-DNA hybridization using the microdilution plate method. The present DNA-DNA hybridization method could identify 29 of 35 strains of lactic acid bacteria isolated from salted vegetables.