APPLICATION OF DIFFERENT IN VIVO POLLINATION TECHNIQUES TO IMPROVE THE FERTILIZATION EFFICIENCY OF INTERSPECIES CROSSES IN THE GENUS PHASEOLUS

摘要

Observations of fertilization in the F_1 of P. vulgaris cv ICA Pijao (Pv) x P. angustissimus PI 535272 (Pa) upon backcrossing with Pv indicated that fertilization occurs 30 or more hours after pollination compared to 8-10 hours in Pv x Pv or Pa x Pa.Abscission of the pods in these backcrosses usually occurs within 3 or 4 days after pollination and embryo develops only to at most a four-cell stage in this time frame (Balasubramanian et al. 2004). A range of pollination techniques including cut-style,grafting and placental pollination have been applied to overcome pre-fertilization barriers in many ornamental crops. The effect of similar alternative in vivo pollination techniques on reducing the time to fertilization in Phaseolus interspecies hybrids was studied.