Investigation of microstructure of bone tissue in mandibles of newborn rats after maternal treatment with antiretroviral drugs

摘要

High-resolution imaging has become a powerful tool for measurements in clinics, laboratory and animal studies, etc. In the present study, we aimed to investigate age related changes in bone development, and the effect of two antiretroviral agents (zidovudine and indinavir), which were administered during pregnancy, on the microstructure and bone mineral density (BMD) in newborn rats (7-, 14- and 28-day-old), with the use of X-ray microcomputed tomography (XMT). Fifty-four mandible bones were collected and divided into 3 groups: group 1 and 2: newborns after maternal treatment of zidovudine and indinavir respectively, group 3: control animals. The specimens were XMT scanned with the resolution of 7 mu m and with a density phantom. Histomorphometric parameters and BMD were calculated to assess bone development depending on the administered drug. A statistical analysis was carried out to compare the differences among the control, zidovudine and indinavir groups. The analysis of the microstructure revealed disturbances in the development of the bone tissue in newborn rats. Indinavir seems to have a greater impact on bone microstructure than zidovudine. (C) 2014 Nalecz Institute of Biocybemetics and Biomedical Engineering. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.