PRODUCTION OF ALKALINE PROTEASE ENZYMES FROM MARINE ACTINOMYCETES AND ITS ANTIMICROBIAL AND ANTI-INFLAMMATORY STUDIES USING MICE MODEL

摘要

Actinomycetes are gram positive bacteria comprising a group of unicellular microorganisms. Streptomycetes is a well-known antibiotic producer. Marine actinomycetes have emerged as a rich source of novel compounds. Alkaline protease enzyme was isolatedfrom Streptomycetes which has wide applications. In the present study, Streptomycetes were isolated from sediment samples collected from Mandapam coast. Gulf of Mannar and it was characterized. Antimicrobial activity was determined by disc diffusion method for the isolates MS-1 and MS-2 treated against human pathogens such as Salmonella, Klebsiella pneumonia, E.coli and P. fluorescence. MS-1 and MS-2 revealed highest antibacterial alkaline protease activity compared to other isolates. Anti-inflammatorystudies using mice model system were performed, the mice were treated with crude extracts of actinomycetes orally inorder to study anti-inflammation. It was found out that within 10 days the mice found to be cured from inflammation after oral supplementation of crude enzyme extract which indicated these bioactive compounds have analgesic properties.