Analysis of Phytochemical Constituent of the Volatile Oil in the Wedelia biflora by HS-SPME/GC-MS

摘要

To analyze the chemical constituents of the volatile oil in the green peel of Wedelia biflora, the chemical constituents was extracted from the Wedelia biflora by solid-phase microextraction. The chemical constituents of the volatile oil separated and identified by GC-MS. The relative content of each component was determined by area normalization. Seventy-two kinds of components were separated. Among them, 68 components were identified, accounting ca. 98,51 % of the total chemical constituents, most of them belonged to sesquiterpenoids, the main chemical constituents of the Wedelia biflora are D-limonene (15.40 %); 4,1 l,ll-trimethyl-8-methylene-flR-(1R~*,4Z,9S~*)]-bicyelo[7.2.0]undee-4-ene (9.70'%); 1R-α-Pinene (8.94 %); 4-methyl-1-(1-methylethyl)-didehydro derive bicyclo[3.1.0]hexane (8.07 %); 1 -methyl-3- (1 -methylethyl)-benzene (6.71 %); 1,1,4,8-tetramethyl- cis, cis, cis-4,7,10- cycloundecatriene (5.39 %); germacrene D (4.65 %), etc. The result of the experiments can provide reference for further research on the chemical components of Wedelia biflora volatile oil as well as for the exploration and utilization of it.