SAW Sensor System for Marker-Free Molecular Interaction Analysis

摘要

A chip-based biosensor system for marker-free detection of affinity and molecular interaction kinetics was developed.It applies surface-acoustic-waves.Combined with a novel double-frequency measurement-mode,which assures reliable reference measurements,it allows a precise real-time detection of mass loading and viscosity alteration.The system is equipped with five independently working channels,which enable it to simultaneously perform different,e.g.,reference measure-ments.Exemplarily performed investigations of the association of thrombin and anti-thrombin antibodies demonstrated the applicability of the system to affinity and kinetic analyses.The system yielded a mean value for the equilibrium dissociation constant of K_D =116 +9nM,which compares well with the supplier specification.