Cloud Point Extraction of Serum Albumin and Its Spectrophotometric Determination in Serum Samples

摘要

A new method based on the cloud point extraction (CPE)separation and ultra-violet spectrometry determination was proposed for the determination of albumin.When the system temperature is higher than the cloud point extraction temperature (CPT)of the mixed surfactant of p-octyl polyethyleneglycolphenyether (Triton X-100)and sodium dodecyl sulfate (SDS),serum albumin could be extracted into surfactant-rich phase.The main factors affecting the cloud point extraction were investigated systematically.Under the optimized conditions,the determination limit for serum albumin as low as 0.18mu g/mL was obtained by preconcentrating a 10mL sample solution,and the relative standard deviation (n =10,c =40.0 mu g/mL)was 3.77%.The proposed method was applied to the determination of albumin in serum samples.The results obtained by this method were in good agreement with coomassie brilliant blue (CBB).