Qualitative and quantitative validation of the microbiological survey method for Listeria spp., Salmonella spp., Enterobacteriaceae and Staphylococcus aureus in food samples.

摘要

The objective of the this study was the preliminary validation of the qualitative MBS method for Salmonella spp. and Listeria spp. and the quantitative microbiological survey (MBS) method for Enterobacteriaceae and Staphylococcus aureus. The MBS method is a rapid alternative method for the detection and selective counting of bacteria in food, water and environmental samples. It is based on a colorimetric survey in single-use disposable reaction vials that can be filled with the samples without any preliminary treatment, such as homogenization or dilution; the greater the number of bacteria present in the samples, the faster the colour change. For qualitative validation of the MBS method for Salmonella spp. and Listeria spp., selectivity, relative accuracy, relative specificity and relative sensitivity were determined. Selectivity, linearity and accuracy were analysed for the quantitative validation of the MBS method for Enterobacteriaceae and S. aureus. The MBS method for Listeria spp., Salmonella spp., Enterobacteriaceae and S. aureus give similar results, which were in agreement with those obtained using the reference method.