Effect of cultivar, type and age of explants, light conditions and plant growth regulators on callus formation of anthurium.

摘要

This paper investigate the effect of cultivar, type and age of explants, light conditions and plant growth regulators (NAA and BA) on callus formation of Anthurium andreanum Linden cultivars Casino and Antadra, an ornamental species. Results showed that the explants source and age, growth conditions, type of cultivar and different concentrations of growth regulators significantly influenced on callus production. Segments of lamina and petiole (micro-cuttings or explants) were cultured in MS basal medium with different concentrations of NAA (0.01, 0.1, 0.5, 1 and 2 mg/L) and BA (0.5, 1, 2 and 3 mg/L) to produce callus. After 65 days, high frequency of callus (0.74 g) was obtained from lamina segments, when they were cultured in MS medium containing 0.5 mg/L NAA+3 mg/L BA in dark conditions. Callus development was observed along the cut margins and midrib regions of the lamina explants. Younger explants grown in dark conditions exhibited better responses to callusing than that of older explants and grown in light conditions. Callus production was better in Antadra than that of Casino. For explants types, permanent callus was produced on the lamina explants, not on the petiole explants.