Optimization of Tissue Culture of Thymus daenensis Celak

摘要

In order to optimization of thyme (Thymus daenensis Celak.) tissue culture, an experi conducted in Estahban Branch, Isalmic Azad University. The experiment designed in factorial experimen on completely randomized design with four replication in MS medium containing BAP (0,0.5,1 and 2 m 2,4-D (0, 0.5, 1 and 2 mg/l) alone or together. In preliminary experiment, the use of 0.5, 1 and 2 mg/l B increased shoot length, number of shoots and leaf number. The use of 0.5 mg/l BAP and 0.5 mg/l 2,4-highest callus formation percentage. In final stage, combined use of 0.5 mg/l BAP and 0.5 mg/l 2,4-increasing of number of shoots and shoot length. Also, the use of 1 mg/l BAP and non-use of 2,4-D, callus formation. In this situation, the increase BAP concentration to 1 mg/l and use of 2,4-D in concentration led to decreasing in studied traits values.