Isozymes and RAPD markers to identify the parenthood of Japanese pear 'Kuratsuki'.

摘要

S (self incompatibility) genotypes, isoenzyme phenotypes and RAPD markers were investigated to identify the parentage of the Japanese pear (Pyrus pyrifolia) cultivar Kuratsuki, which was derived from a chance seedling found in a mixed planting of cultivars Shinsui, Kosui, Hosui and Chojuro. The S genotype of Kuratsuki was judged to be S3S5, the same as that of Hosui, in that the pollen of Kuratsuki was cross-incompatible, and it exhibited the same PCR-RFLP pattern for the S-RNase [ribonuclease] genes as Hosui. Analyses of 7 loci for 5 isoenzymes among the parental candidates showed that Kuratsuki shared the common a allele at the POD-1 (peroxidase) locus with Shinsui and Chojuro, and the common b allele at the PGM-3 (phosphoglucomutase) locus onlywith Hosui. The common RAPD markers specific to Kuratsuki were detected only in Shinsui and Hosui. The results suggest that Kuratsuki is a hybrid from a cross between Shinsui and Hosui.