Determination of trace amounts of nystatin in water and vaccine samples using sodium dodecyl sulfate-coated iron oxide magnetic nanoparticles followed by high-performance liquid chromatography-ultraviolet detection

摘要

In this work, magnetic solid-phase extraction based on sodium dodecyl sulfate-coated Fe3O4 nanoparticles has been successfully applied for extraction and preconcentration of trace amounts of nystatin from water and vaccine samples prior to high-performance liquid chromatography-ultraviolet detection. Various experimental parameters affecting extraction and recovery of the analyte, such as the amount of sodium dodecyl sulfate, pH of the sample solution, salt concentration, extraction time, sample volume and desorption conditions, were systematically studied and optimized. Under optimized conditions, nystatin was quantitatively extracted. Proper linear range with good coefficient of determination, (R (2) > 0.99) and limit of detection and quantification (based on signal-to-noise ratios of 3 and 10) of 2.0 and 5.0 A mu g L-1, over the investigated concentration range (5-700 A mu g L-1), were obtained, respectively. The intra-day and inter-day relative standard deviations at 50 A mu g L-1 level of NYS were 1.4 and 4.5% based on six replicate determinations. The accuracy of the method was evaluated by recovery measurements on spiked samples. Suitable recoveries of 96-102 and 26-44% were achieved (at spiked levels of 50, 300 and 500 A mu g L-1) for water and vaccine samples, respectively.