Design, synthesis and in vivo Iin vitro screening of novel chlorokojic acid derivatives

摘要

A series of novel Mannich bases of chlorokojic acid (2-chloromethyl-5-hydroxy-4H-pyran-4-one) were synthesized and their biological activities were investigated. Anticonvulsant activity results according to phase-l tests of Antiepileptic Drug Development (ADD) Program revealed that compound 13 was the most effective one at 4h against subcutaneous pentylenetetrazole (scPTZ)-induced seizure test. Antimicrobial activities were evaluated in vitro against bacteria and fungi by using broth microdilution method.The antitubercular activities against Mycobocterium tuberculosis and M. avium were discussed with Resazurin microplate assay (REMA).The antimicrobial activity results indicated that compounds , 12 (MIC: 8-16 ug/mL) showed higher activity against Gram negative bacteria while compound 12 had MIC: 4-16 ug/mL against Gram positive bacteria. Compound 1 was the most active one with MIC values of 8-32 ug/mL against fungi. Mannich bases also exhibit significant antitubercular activity in a MIC range of 4 to 32 ug/mL, especially compound 18 against M. avium.