Quantification of amlodipine in dried blood spot samples by high performance liquid chromatography tandem mass spectrometry

摘要

Highlights?An HPLC–MS/MS method for determination of amlodipine in dried blood spots has been developed.?This validated method proved to be sensitive and specific for amlodipine measurement.?The method is being used in detecting amlodipine in patient samples in a large pharmacoepidemiology study.?The method could potentially be applied in medication adherence assessment in patients prescribed amlodipine.AbstractA sensitive and specific method, utilising high performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS) was developed for the quantitative determination of amlodipine in dried blood spot (DBS) samples. Chromatographic separation was achieved using a Waters XBridge C18 column with gradient elution of a mixture of water and acetonitrile containing 0.1% formic acid (v/v). Amlodipine was quantified using a Waters Quattro Premier mass spectrometer coupled with an electro-spray ion