Characterization and molecular evolution ofclaudingenes in thePungitius sinensis

摘要

Claudins are a family of integrated membrane-bound proteins involving in paracellular tightness, barrier forming, ion permeability, and substrate selection at tight junctions of chordate epithelial and endothelial cells. Here, 39 putativeclaudingenes were identified in thePungitius sinensisbased on the high throughput RNA-seq. Conservative motif distribution in each group suggested functional relevance. Divergence of duplicated genes implied the species' adaptation to the environment. In addition, selective pressure analyses identified one site, which may accelerate functional divergence in this protein family. Pesticides cause environmental pollution and have a serious impact on aquatic organisms when entering the water. The expression pattern of mostclaudingenes was affected by organophosphorus pesticide, indicating that they may be involved in the immune regulation of organisms and the detoxification of xenobiotics. Protein-protein network analyses also exhibited 439 interactions, which implied the functional diversity. It will provide some references for the functional study onclaudingenes.