An Efficient Approach for Large-Scale Production of Sialyglycopeptides from Egg Yolks

摘要

Glycopeptides are very useful substrates for the synthesis of various complex glyco-proteins and glycopeptides that are indispensable materials for the structural ana functional investigation of glycoproteins. This report provides an efficient and time-and cost-saving strategy for the large-scale production of a sialylglycopeptide (SGP) that carries an intact N-linked disialyl-biantennary complex-type sialylohgosaccharide. Consequently, 680 mg of SGP was obtained from 100 egg yolks after phenol treatment, Sephadex G-50 gel filtration, and filtration through graphitized carbon cartridges. The chemical structure and the glycosylation site of the isolated SGP were identified by NMR and ESI-MS/MS, and the purity measured with HPLC was over 95%.