Identification of the Genes Involved in Cotton Induced Defense to Attack of Cotton Aphids (Aphis gossypii)

摘要

Cotton aphid (Aphis gossypii Glover) is one of the most important economic pests in the world. As a result of long-term unreasonable use of insecticides, cotton aphids have produced resistance to many insecticides which lead to the serious occurrence of cotton aphids in many regions. In the case, it is an urgent task to work actively on the development and cultivation of aphid-resistant crop varieties using plant resistance. We constructed the SSH library of cotton leaves infested by cotton aphids with SSH (Suppression subtractive hybridization) method. A total of 253 independent clones were analyzed from the SSH library. The size of the clones varied from 250 to 2000 bp. The clones were sequenced and the expressed sequence tags (ESTs) were analyzed through homology searches. From opinion of stress, there were 38 clones on biotic stress, 95 clones on abiotic stress, 93 clones on biotic and abiotic stress, and 27 clones on others. The expressed levels of five genes from the SSH library showed certain changes respectively under cotton aphid attack and short time wounding induction by qRT-PCR (Quantitative real-time polymerase chain reaction). These results suggested that cotton induced defense of cotton aphids is relatively complex and involved several signal transduction pathway.