Multiresidue Method for Quantification of Sulfonamides and Trimethoprim in Tilapia Fillet by Liquid Chromatography Coupled to Quadrupole Time-of-Flight Mass Spectrometry Using QuEChERS for Sample Preparation

摘要

A multiresidue method for detecting and quantifying sulfonamides (sulfapyridine, sulfamerazine, sulfathiazole, sulfamethazine, sulfadimethoxine, sulfamethoxazole, and sulfamethoxypyridazine) and trimethoprim in tilapia 7 llet (Oreochromis niloticus) using liquid chromatography coupled to mass spectrometry was developed and validated.. e sample preparation was optimized using the QuEChERS approach.. e chromatographic separation was performed using a C18 column and 0.1% formic acid in water and acetonitrile as the mobile phase in the isocratic elution mode. Method validation was performed based on the Commission Decision 2002/657/EC and Brazilian guideline.. e validation parameters evaluated were linearity (r = 0.99); limits of detection (LOD) and quanti 7 cation (LOQ), 1 ng center dot g(-1) and 5 ng center dot g(-1), respectively; intraday and interdays precision (CV lower than 19.4%).. e decision limit (CC alpha 102.6-120.0 ng center dot g(-1) and 70 ng center dot g(-1) for sulfonamides and trimethoprim, respectively) and detection capability (CC ss 111.7-140.1 ng center dot g(-1) and 89.9 ng center dot g(-1) for sulfonamides and trimethoprim, respectively) were determined. Analyses of tilapia 7 llet samples from 7 sh exposed to sulfamethazine through feed (incurred samples) were conducted in order to evaluate the method.. is new method was demonstrated to be fast, sensitive, and suitable for monitoring sulfonamides and trimethoprim in tilapia 7 llet in health surveillance programs, as well as to be used in pharmacokinetics and residue depletion studies.