Detection of Antibody Against Avian Leukosis Virus Subgroup A by Enzyme-linked Immunosorbent Assay with BaculovirusExpressed gp85 Protein

摘要

To detect antibody against avian leukosis virus subgroup A (ALV-A), the gp85 gene of an ALV-A strain isolated in Taiwan was cloned and expressed in a baculovirus expression vector. The expressed protein confirmed by Western blotting and immunofluorescence tests with anti-ALV-A antiserum was coated in 96-well plates and was used as an antigen for antibody detection by an indirect enzyme-linked immunosorbent assay (ELISA). Neutralization test performed in chicken sera from the infected and uninfected flocks was used as the standard to evaluate the accuracy of the ELISA. The cut-off point was calculated by receiver operator characteristic (ROC) curve to be 1. The sensitivity and specificity of this ELISA were 80% (24/30) and 83.3% (35/42), respectively. In conclusion, this ELISA could be applied for the anti-ALV-A antibody detection in the field.