Mesotrione herbicide does not cause genotoxicity, but modulates the genotoxic effects of Atrazine when assessed in mixture using a plant test system (Allium cepa)

摘要

Mesotrione (MES) is an herbicide from the triketone family and has been used as an alternative to Atrazine (ATZ), which was banned in some countries due to its toxicity to non-target organisms. Despite being considered an eco-friendly herbicide, data from the literature about the harmful effects of MES in its pure form and/or in combination with other herbicides is still scarce. Aimed at assessing the potential of MES to induce cell death and DNA damage, seeds of Allium cepa (higher plant, monocotyledon) were exposed to this herbicide, pure and in mixture with ATZ, and the number of dividing cells (cytotoxicity), chromosomal aberrations (CA, genotoxicity) and micronuclei (MN, mutagenicity) were then quantified. The pure MES (1.8 to 460 mu g/L) did not show either cytotoxicity or genotoxicity/mutagenicity under the tested conditions. The genotoxicity of ATZ (1.5 to 400 mu g/L), previous reported in the literature, was confirmed herein. The assessment of MES + ATZ mixtures (1.8 + 1.5; 7 + 6.25; 30 + 25 mu g/L, respectively) showed that MES, at low concentrations, enhance the genotoxicity of ATZ (potentiation), since the significant frequencies of CA and MN were greater than the ones expected in additive effects. Taking together, MES in its pure form seems to be a safe alternative to ATZ regarding the capacity to damage (at cellular and DNA levels) non-target plants (Monocots); however, MES in combination with ATZ appeared to act as a co-mutagen at low concentrations.