Improving the accuracy of pancreatic cancer clinical staging by exploitation of nanoparticle-blood interactions: A pilot study

摘要

BackgroundPancreatic ductal adenocarcinoma (PDAC) early diagnosis is? crucial ?and new, cheap and user-friendly techniques for biomarker identification? are ?needed. “Protein corona” (PC) is emerging a new bio-interface potentially useful in tumor early diagnosis. In a previous investigation, we showed that relevant differences between the ?protein patterns of? PCs formed on lipid NPs after exposure to PDAC and non-cancer plasma? samples exist. To extend that research, We performed this pilot study to investigate the effect of PDAC tumor size and distant metastases on PC composition. MethodsTwenty PDACs were clinically staged according to the UICC TNM staging system 8?t?h?Edition. Collected plasma samples were let to interact with lipid NPs; resulting PCs were characterized by SDS-PAGE. To properly evaluate changes in the PC, the protein intensity profiles were reduced to four regions of molecular weight: 120?kDa.? ResultsData analysis allowed to?distinguish T1-T2 cases from T3 and?above all from?metastatic ones (p?