Two validated spectrofluorimeteric and high performance liquid chromatography (HPLC) methods with fluorescence detection for the analysis of a new anti‐hepatitis C drug, daclatasvir hydrochloride, in raw material or tablet form and in biological fluids

Aboshabana Rasha; Shalan Shereen; Eid Manal; El‐Enany Nahed

摘要

Abstract > Two sensitive and accurate methods have been developed for the estimation of daclatasvir (DAC) in its raw material, dosage form and in biological fluids. Method I is based on the measurement of DAC native fluorescence in methanol at 385?nm after excitation at 315?nm. The relationship between fluorescence intensity and concentration was found to be rectilinear over the linearity range (3.0–30.0?ng/ml). There were good per cent recoveries both in the dosage form (99.87?±?0.84) and in spiked human plasma (99.96?±?1.54%). Method II utilized reversed‐phase high performance liquid chromatography to estimate the antiviral agent daclatasvir hydrochloride against hepatitis C within 4.0?min on a C18 column (Eurosphere. 100‐5 C18, 150?×?4.6?mm, Germany) using a mobile phase consisting of acetonitrile and 0.1?M sodium dihydrogen phosphate (30:70, v/v) at pH?3.0 and with a fluorescence detector adjusted to 315?nm and 385?nm for excitation and emission respectively. The calibration curve was linear over the range (20.0–200.0?ng/ml) with SD 1.38%, error 0.56%, recovery 99.99?±?1.30% in tablets, recovery 100.28?±?1.73% in spiked urine and recovery 99.63?±?2.72% in spiked plasma.The new developed methods were successfully applied to the assay of the daclatasvir in tablet form and extended to its determination in real plasma, spiked human plasma and urine. The analytical performance of the proposed method was validated according to International Conference on Harmonization (ICH) guidelines. The proposed methods were compared with the results of a comparison method and it was found that there was no significant difference between the methods, as revealed by Student's t ‐test and variance ratio F‐test. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >已经开发了两个敏感和准确的方法，以估计Daclatasvir（DAC）的原始材料，剂型和生物液中的材料。方法I基于在315℃的激发后在385℃下在385℃下测量甲醇的DAC原生荧光。发现荧光强度和浓度之间的关系在线性范围（3.0-30.0〜Ng / ml）上是直线性的。剂量形式的百分之次恢复（99.87？±0.84）和尖刺人血浆（99.96？±1.54％）。方法II利用反相高效液相色谱法以在C18柱（EuroSphere）的4.0℃内估计盐酸抗病毒剂DaclataSvir盐酸盐逆型丙型肝炎（EuroSphere。100-5 C18,150？×4.6？mm，德国）使用流动阶段由pHβ3.0和荧光检测器以乙腈和0.1·m磷酸二氢钠（30:70，v / v）组成，分别调节至315〜315〜385Ω·nm。校准曲线随着SD 1.38％的范围（20.0-200.0〜Ng / ml）线性，误差0.56％，恢复99.99？±1.30％，恢复100.28？±1.73％在尖刺尿液中，回收99.63？旋转等离子体中的2.72％。新的开发方法成功地应用于片剂形式的Daclatasvir的测定，并延伸到真实血浆中的测定，尖刺的人血浆和尿液。根据协调（ICH）指南的国际会议，验证了该方法的分析性能。将所提出的方法与比较方法的结果进行比较，发现该方法之间没有显着差异，如学生的 T </ i> - 差异和方差比F检验所透露。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-26441/'>《Luminescence: The journal of biological and chemical luminescence》</a> <b style="margin: 0 2px;">|</b><span>2018年第8期</span><b style="margin: 0 2px;">|</b><span>共13页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Aboshabana Rasha&option=202" target="_blank" rel="nofollow">Aboshabana Rasha;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shalan Shereen&option=202" target="_blank" rel="nofollow">Shalan Shereen;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Eid Manal&option=202" target="_blank" rel="nofollow">Eid Manal;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=El‐Enany Nahed&option=202" target="_blank" rel="nofollow">El‐Enany Nahed;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Analytical Chemistry Faculty of PharmacyUniversity of MansouraMansoura Egypt;</p> <p>Department of Analytical Chemistry Faculty of PharmacyUniversity of MansouraMansoura Egypt;</p> <p>Department of Analytical Chemistry Faculty of PharmacyUniversity of MansouraMansoura Egypt;</p> <p>Department of Analytical Chemistry Faculty of PharmacyUniversity of MansouraMansoura Egypt;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1345.html" title="生物光学">生物光学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biological fluids&option=203" rel="nofollow">biological fluids;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=daclatasvir (DAC)&option=203" rel="nofollow">daclatasvir (DAC);</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fluorescence detection&option=203" rel="nofollow">fluorescence detection;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=HPLC&option=203" rel="nofollow">HPLC;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=method validation&option=203" rel="nofollow">method validation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=real plasma&option=203" rel="nofollow">real plasma;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=spectrofluorimetry&option=203" rel="nofollow">spectrofluorimetry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=tablets&option=203" rel="nofollow">tablets;</a> </p> <div class="translation"> 机译：生物液;Daclatasvir（DAC）;荧光检测;HPLC;方法验证;真实等离子体;光谱氟状物;平板电脑; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023986118.html">Two validated spectrofluorimeteric and high performance liquid chromatography (HPLC) methods with fluorescence detection for the analysis of a new anti‐hepatitis C drug, daclatasvir hydrochloride, in raw material or tablet form and in biological fluids</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Aboshabana Rasha&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Aboshabana Rasha,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shalan Shereen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Shalan Shereen,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Eid Manal&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Eid Manal,</a> <a href="/journal-foreign-26441/" target="_blank" rel="nofollow" class="tuijian_authcolor">Luminescence: The journal of biological and chemical luminescence .</a> <span>2018</span><span>,第8期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：具有荧光检测的两种验证的光谱氟位测液和高效液相色谱（HPLC）方法，用于分析新的抗丙型肝炎药物，盐酸Daclatasvir盐酸盐，原料或片剂形式和生物流体</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070403120332.html">Fast and Environmentally Friendly Quantitative Analysis of Active Agents in Anti-Diabetic Tablets by an Alternative Laser-Induced Breakdown Spectroscopy (LIBS) Method and Comparison to a Validated Reversed-Phase High-Performance Liquid Chromatography (RP -HPLC) Method</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Victor Ulises Contreras&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Victor Ulises Contreras,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Marco A. Meneses-Nava&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Marco A. Meneses-Nava,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nancy Ornelas-Soto&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Nancy Ornelas-Soto,</a> <a href="/journal-foreign-19376/" target="_blank" rel="nofollow" class="tuijian_authcolor">Applied Spectroscopy: Society for Applied Spectroscopy .</a> <span>2012</span><span>,第11期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过替代的激光诱导击穿光谱法（LIBS）和与已验证的反相高效液相色谱法（RP -HPLC）进行比较，快速，环保地对抗糖尿病片剂中的活性剂进行定量分析</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704020638592.html">Correlation analysis based on the hydropathy properties of non-steroidal anti-inflammatory drugs in solid-phase extraction (SPE) and reversed-phase high performance liquid chromatography (HPLC) with photodiode array detection and their applications to biological samples</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Milanetti Edoardo&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Milanetti Edoardo,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Carlucci Giuseppe&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Carlucci Giuseppe,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Olimpieri Pier Paolo&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Olimpieri Pier Paolo,</a> <a href="/journal-foreign-34102/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of chromatography, A: Including electrophoresis and other separation methods .</a> <span>2019</span><span>,第期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于具有光电二极管阵列检测的非甾体抗炎药中的非甾体抗炎药水小肿性能的相关分析及其对生物样品的应用</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-256173_thesis/0705011038238.html">Analytical Method Development for Polymeric Materials in Biological Fluids Using High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometric Detection (HPLC-MS/MS)</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Changyu Quang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Changyu Quang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nichole R. Myers&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Nichole R. Myers,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=William C. Nethero&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">William C. Nethero,</a> <a href="/conference-foreign-256173/" target="_blank" rel="nofollow" class="tuijian_authcolor">ASMS Conference on Mass Spectrometry and Allied Topics .</a> <span>2014</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用高效液相色谱法与串联质谱检测耦合（HPLC-MS / MS）的生物流体中聚合物材料的分析方法</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061381377.html">Part I. Rapid analysis of pharmaceutical compounds using high-speed HPLC columns. Part II. Simulataneous determination of drugs in tablet dosage forms using packed column supercritical fluid chromatography. Part III. Pharmaceutical analysis of selected drugs using capillary electrophoresis.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhou, Meng.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhou, Meng. </a> <span>2001</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：第一部分：使用高速HPLC色谱柱对药物化合物进行快速分析。第二部分使用填充柱超临界流体色谱法同时测定片剂剂型中的药物。第三部分使用毛细管电泳对选定药物进行药物分析。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040006097360.html">Single Laboratory Validation of a Method for Determination of Glucosamine in Raw Materials and Dietary Supplements Containing Glucosamine Sulfate and/or Glucosamine Hydrochloride by High-Performance Liquid Chromatography with FMOC-Su Derivatization</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Joseph ZiQi Zhou&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Joseph ZiQi Zhou,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ted Waszkuc&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ted Waszkuc,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Felicia Mohammed&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Felicia Mohammed </a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：FMOC-Su衍生化高效液相色谱法测定含硫酸葡萄糖胺和/或盐酸葡萄糖胺的原料和膳食补充剂中葡萄糖胺的方法的单实验室验证</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100098770393.html ">Development and validation of the analytical method by high performance liquid chromatography (HPLC) for Lamotrigine raw material Development and validation of the analytical method by high performance liquid chromatography (HPLC) for Lamotrigine raw material</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hisao Nishijo&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hisao Nishijo,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Keyla Carla de Freitas Pereira&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Keyla Carla de Freitas Pereira,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guilherme Nobre Lima do Nascimento&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guilherme Nobre Lima do Nascimento,</a> <span>2011</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：拉莫三嗪原料的高效液相色谱（HpLC）分析方法的开发和验证拉莫三嗪原料的高效液相色谱（HpLC）分析方法的开发和验证</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/ntis-science-report_pb_thesis/020711340140.html">Methods Development for the Analysis of Catecholamines and Indoleamines with the Use of HPLC (High Performance Liquid Chromatography) Combined with Electrochemical Detection</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Newport, G. D. &option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Newport, G. D. ,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Slikker, W.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Slikker, W. </a> <span>1982</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：方法利用高效液相色谱法（高效液相色谱法）结合电化学检测技术开发儿茶酚胺和吲哚胺的分析方法</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130437612466.html">method for the simultaneous determination of purine derivatives and pirimidine, amino acid n - acetylated and dicarbossilici acids in a biological sample by high-performance liquid chromatography (hplc) and high-performance on kit.</a> <b>[P]</b> . <span> 外国专利：  ITRM20050085A1 </span> <span> . 2006-09-01</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：高效液相色谱法（hplc）和高性能试剂盒同时测定生物样品中嘌呤衍生物和嘧啶，氨基酸正乙酰化和二碳碳硅酸的方法 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130500883564.html">SYSTEMS, MATERIALS, AND METHODS FOR REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (RP-HPLC) FOR MONITORING FORMATION OF MULTI-SPECIFIC MOLECULES</a> <b>[P]</b> . <span> 外国专利：  WO2021209953A1 </span> <span> . 2021-10-21</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于监测多特异性分子形成的逆相高性能液相色谱（RP-HPLC）的系统，材料和方法 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130419170237.html">Switchable fluidic valve for sample separating apparatus used in high-performance liquid chromatography (HPLC) system, is switched to diagnostic switching position in which leakage of fluid channels to fluid paths is diagnosed</a> <b>[P]</b> . <span> 外国专利：  DE102011087935A1 </span> <span> . 2013-06-13</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：高性能液相色谱（HPLC）系统中使用的用于样品分离设备的可切换流体阀切换到诊断切换位置，在此位置诊断出流体通道向流体路径的泄漏 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704023986118','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704023986118down" data-source="7," data-out-id="v5WYvPjKObKo2x09u0zhCBTsVCCmZWErst68M3FvanTNUvIMiDzdyXDYU6rvv7n4," data-f-source-id="7" data-title="Two validated spectrofluorimeteric and high performance liquid chromatography (HPLC) methods with fluorescence detection for the analysis of a new anti‐hepatitis C drug, daclatasvir hydrochloride, in raw material or tablet form and in biological fluids" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="26441" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Two validated spectrofluorimeteric and high performance liquid chromatography (HPLC) methods with fluorescence detection for the analysis of a new anti‐hepatitis C drug, daclatasvir hydrochloride, in raw material or tablet form and in biological fluids"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.8"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.8"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>