Triarylmethyl-based biradical as a superoxide probe

摘要

Superoxide radical represents one of the most biologically relevant reactive oxygen species involved in numerous physiological and pathophysiological processes. Superoxide measurement through the decay of an electron paramagnetic resonance (EPR) signal of a triarylmethyl (TAM) radical possesses the advantage of a high selectivity and relatively high rate constant of TAM reaction with the superoxide. Hereby we report a straightforward synthesis and characterization of a TAMTAM biradical showing a high reactivity with superoxide (second-order rate constant, (6.7 ?0.2) ?103 M1 s1) enabling the measurement of superoxide radical by following the increase of a sharp EPR signal associated with the formation of a TAM-quinone-methide monoradical product. ?2017 Informa UK Limited, trading as Taylor & Francis Group.