Sensitive quantification of BB-22 and its metabolite BB-22 3-carboxyindole, and characterization of new metabolites in authentic urine and/or serum specimens obtained from three individuals by LC-QTRAP-MS/MS and high-resolution LC-Orbitrap-MS/MS

摘要

PurposeA synthetic cannabinoid BB-22 and its metabolite BB-22 3-carboxyindole have not yet been quantifiedin human urine. The aim of this study is to establish a sensitive analytical method for the quantification of BB-22 and its 3-carboxyindole in human serum and urine specimens, and the characterization of the unreported metabolites of BB-22 in authentic urine specimens from three individuals.MethodsThese compounds were extracted from -glucuronide-hydrolyzed and unhydrolyzed urine and/or serum via liquid-liquid extraction. The identification and quantification were performed using liquid chromatography (LC)-QTRAP-tandem mass spectrometry (MS/MS) and the characterization of the new metabolites was made by high-resolution LC-MS/MS.ResultsThe limits of detection of BB-22 and BB-22 3-carboxyindole were 3 and 30pg/mL in urine, respectively. The devised method was applied to quantify these compounds in authentic serum and urine obtained from two drug abusers and in urine from one drug abuser. The serum levels of BB-22 were 149 and 6680pg/mL, and those of BB-22 3-carboxyindole were 0.755 and 38.0ng/mL in cases 1 and 2, respectively. The urine levels of BB-22 were 5.64, 5.52 and 6.92pg/mL and those of BB-22 3-carboxyindole were 0.131, 21.4 and 5.15ng/mL in cases 1, 2 and 3, respectively. New monohydroxyl metabolites retaining the structure of BB-22 were found in the urine specimens.ConclusionsThe synthetic cannabinoid BB-22 and its metabolite BB-22 3-carboxyindole were identified and quantified in authentic human serum and urine specimens for the first time, and new metabolites of BB-22 were tentatively identified in authentic urine specimens obtained from three drug usersin this study.