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Th17 cells differentiated with mycelial membranes of Candida albicans prevent oral candidiasis

机译:与念珠菌蛋白酶菌丝膜分化的Th17细胞免受口腔念珠菌病

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摘要

Candida albicans is a human commensal that causes opportunistic infections. Th17 cells provide resistance against mucosal infection with C. albicans; however, the T cell antigens remain little known. Our final goal is to find effective T cell antigens of C. albicans that are responsible for immunotherapy against candidiasis. Here, we prepared fractions including cytosol, membrane and cell wall from yeast and mycelial cells. Proteins derived from a membrane fraction of mycelial cells effectively induced differentiation of CD4(+) T cells into IL-17A-producing Th17 cells. To confirm the immunological response in vivo of proteins from mycelial membrane, we performed adoptive transfer experiments using ex vivo stimulated CD4(+) T cells from IL-17A-GFP reporter mice. Mycelial membrane-differentiated CD4(+) Th17 cells adoptively transferred intravenously prevented oral candidiasis by oral infection of C. albicans, compared with control anti-CD3-stimulated CD4(+ )T cells. This was confirmed by the clinical score and the number of neutrophils on the infected tissues. These data suggest that effective T cell antigens against candidiasis could be present in the membrane protein fraction of mycelial cells. The design of novel vaccination strategies against candidiasis will be our next step.
机译:念珠菌白名单是一种引起机会性感染的人类共生。 Th17细胞提供抗粘膜感染的粘膜感染抗白醛。然而,T细胞抗原仍然很少知道。我们的最终目标是寻找对念珠菌病的免疫疗法有效的C. albicans的有效T细胞抗原。在这里,我们制备了来自酵母和菌丝细胞的细胞溶溶胶,膜和细胞壁的馏分。衍生自菌丝细胞的膜级分的蛋白质有效地诱导了CD4(+)T细胞的分化为IL-17A产生的TH17细胞。为了确认菌丝膜的蛋白质体内的免疫应答,我们使用来自IL-17A-GFP报告小鼠的EXVivo刺激的CD4(+)T细胞进行了采用的转移实验。与对照抗CD3刺激的CD4(+)T细胞相比,菌丝膜分化的CD4(+)Th17细胞通过口服感染静脉内预防口服念珠菌病。这是通过临床评分和感染组织上的中性粒细胞数来证实。这些数据表明,对念珠菌病有效的T细胞抗原可能存在于菌丝细胞的膜蛋白分数中。针对念珠菌病的新型疫苗接种策略的设计将是我们的下一步。

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  • 来源
    《FEMS Yeast Research》 |2018年第3期|共10页
  • 作者单位

    Fukuoka Dent Coll Dept Funct Biosci Sect Infect Biol Sawara Ku 2-15-1 Tamura Fukuoka Fukuoka 8140193 Japan;

    Fukuoka Dent Coll Dept Funct Biosci Sect Infect Biol Sawara Ku 2-15-1 Tamura Fukuoka Fukuoka 8140193 Japan;

    Fukuoka Dent Coll Dept Funct Biosci Sect Infect Biol Sawara Ku 2-15-1 Tamura Fukuoka Fukuoka 8140193 Japan;

    Fukuoka Dent Coll Dept Funct Biosci Sect Infect Biol Sawara Ku 2-15-1 Tamura Fukuoka Fukuoka 8140193 Japan;

    Fukuoka Dent Coll Dept Funct Biosci Sect Infect Biol Sawara Ku 2-15-1 Tamura Fukuoka Fukuoka 8140193 Japan;

    Fukuoka Dent Coll Adv Sci Res Ctr Sawara Ku 2-15-1 Tamura Fukuoka Fukuoka 8140193 Japan;

    Fukuoka Dent Coll Dept Funct Biosci Sect Infect Biol Sawara Ku 2-15-1 Tamura Fukuoka Fukuoka 8140193 Japan;

    Fukuoka Dent Coll Dept Funct Biosci Sect Infect Biol Sawara Ku 2-15-1 Tamura Fukuoka Fukuoka 8140193 Japan;

    Fukuoka Dent Coll Dept Oral Growth &

    Dev Sect Dent Disabled Sawara Ku 2-15-1 Tamura Fukuoka Fukuoka 8140193 Japan;

    Fukuoka Dent Coll Dept Funct Biosci Sect Infect Biol Sawara Ku 2-15-1 Tamura Fukuoka Fukuoka 8140193 Japan;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

    Candida albicans; mycelial membranes; Th17; oral candidiasis; adoptive transfer;

    机译:念珠菌蛋白质;菌丝膜;Th17;口腔念珠菌病;养老金;

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