Linolelaidic acid induces apoptosis, cell cycle arrest and inflammation stronger than elaidic acid in human umbilical vein endothelial cells through lipid rafts

摘要

Lipid rafts were reported to play important roles in elaidic acid-induced apoptosis in human umbilical vein endothelial cells (HUVECs). Linolelaidic acid induced cell injury more seriously than elaidic acid. However, whether lipid rafts may play the same roles for linolelaidic acid- and elaidic acid-induced cell injury was unknown. Therefore, the aim of this study was to compare the effect of lipid rafts on linolelaidic acid-and elaidic acid-induced apoptosis, cell cycle arrest and inflammation in HUVECs. The cell viability was significantly decreased, the number of apoptotic cells and cell population in G1 phase were significantly increased in elaidic acid-or linolelaidic acid-treated cells, compared to methylb-cyclodextrin_trans-fatty acids-treated cells. In addition, the expression levels of pro-apoptotic proteins (caspase-3, -8, Bax, p53) and inflammation factors (vascular cell adhesion molecule-1, intercellular adhesion molecule, E-selectin and nitro oxide) were significantly increased, and antiapoptotic protein like Bcl-2 decreased significantly in linolelaidic acid-treated cells, which were stronger than those in elaidic acid-treated cells. In conclusion, linolelaidic acid induces a stronger lesion effect on HUVECs through lipid rafts compared to elaidic acid.