α4 contributes to bladder urothelial carcinoma cell invasion and/or metastasis via regulation of E-cadherin and is a predictor of outcome in bladder urothelial carcinoma patients

摘要

Aim α4 is upregulated in several types of human cancer and possesses an oncogenic role. However, the abnormalities of α4 and its underlying mechanisms in the pathogenesis of bladder urothelial carcinoma (BUC) remain unknown. Methods α4 expression profile was examined by reverse transcription polymerase chain reaction, western blotting and immunohistochemistry (IHC) in BUC tissues and normal urothelial bladder epithelial tissues. Short hairpin RNA (ShRNA) interfering approach was employed to suppress endogenous α4 expression in BUC cells to determine its role in tumourigenesis, invasion/metastasis and the potential mechanism. Results α4 expression in BUC was significantly up-regulated at both mRNA and protein levels compared with that in normal urothelial bladder epithelial tissues. High expression of α4 was inversely correlated with poor survival of the BUC patients (P < 0.05). Down-regulation of α4 in BUC EJ and T24 cells led to a G1 phase cell cycle arrest, suppressed cell growth, markedly inhibited invasive motility in vitro and metastatic potential in vivo. Moreover, down-regulation of α4 was found to increase E-cadherin expression and reduce metastasis-associated protein 1 (MTA1) expression either in transcript or protein levels. Importantly, a significant correlation between high expression of α4 and negative expression of E-cadherin in BUC cohorts was observed (P < 0.001). Conclusions These findings suggest a potential important role of α4 in control of cell migration and/or invasion via the regulation of E-cadherin expression, and the high α4 expression, as examined by IHC, is an independent molecular marker for shortened survival time of patients with BUC.