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An Integrated Real‐time Electrochemical LAMP Device for Pathogenic Bacteria Detection in Food

机译：一种用于食品中致病细菌检测的集成实时电化学灯装置

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Abstract > A novel, integrated, real‐time electrochemical loop‐mediated isothermal amplification (LAMP) device for pathogen detection in food was developed. The electrochemical LAMP device for detection of the vt gene in E. coli O157?:?H7 and invA gene in Salmonella enterica was implemented with DNA intercalating probes‐methylene blue. To improve assay efficiency, the optimal concentration of MB was determined to be 30?μM. The real‐time electrochemical LAMP results of gene serial dilutions were compared to the fluorescence‐based detection method. For E. coli O157 and Salmonella , the assay detection limits were 10 DNA copies and 1 DNA copy, respectively, in a reaction time of less than 30?min. The electrochemical and optical LAMP method analyses were realized with 10?% milk to simulate the turbid environment of real food samples. In real‐time LAMP of Salmonella in a turbid environment, the detection limit of 10 copies without pretreatment was possible using the electrochemical method only. Our electrochemical device was used for bacterial detection in 150 turbid food samples, including juice, milk, and soy milk. This electrochemical device has many advantages, including size, portability, high specificity, and high sensitivity, while also being a user‐friendly device that provides stable signals. Thus, to the best of our knowledge, this new electrochemical device demonstrates the best performance for bacterial detection in the rapid contaminated food detection category. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >一种新颖的，集成，实时电化学环介导等温放大（灯）装置发育食品中的病原体检测。用于检测 e中的 vt </ i>基因的电化学灯装置。 COLI </ i> O157？：ΔH7和 INVA </ I>基因在沙门氏菌中，用DNA插入探针 - 亚甲基蓝色实施。为了提高测定效率，测定Mb的最佳浓度为30Ωμm。将基因系列稀释液的实时电化学灯与基于荧光的检测方法进行比较。对于 e。 Coli </ i> O157和 Salmonella </ i>，测定检测限是10 DNA拷贝和1个DNA拷贝，在少于30Ω·min的反应时间。电化学和光学灯法分析用10倍牛奶实现，模拟真实食物样品的混浊环境。在浑浊环境中的沙门氏菌的实时灯中，使用仅使用电化学方法可以进行10份没有预处理的拷贝的检出限。我们的电化学装置用于150个浑浊食物样品中的细菌检测，包括果汁，牛奶和豆浆。该电化学装置具有许多优点，包括尺寸，便携性，高特异性和高灵敏度，同时也是提供稳定信号的用户友好的设备。因此，据我们所知，这种新的电化学装置证明了快速受污染的食物检测类别中的细菌检测最佳性能。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2018年第10期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huang Tsung‐Tao&option=202" target="_blank" rel="nofollow">Huang Tsung‐Tao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Shao‐Chung&option=202" target="_blank" rel="nofollow">Liu Shao‐Chung;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huang Chih‐Hung&option=202" target="_blank" rel="nofollow">Huang Chih‐Hung;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lin Chun‐Ju&option=202" target="_blank" rel="nofollow">Lin Chun‐Ju;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huang Sheng‐Tung&option=202" target="_blank" rel="nofollow">Huang Sheng‐Tung;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Chemical Engineering and BiotechnologyNational Taipei University of TechnologyTaipei 106 Taiwan;</p> <p>Department of Chemical Engineering and BiotechnologyNational Taipei University of TechnologyTaipei 106 Taiwan;</p> <p>Department of Chemical Engineering and BiotechnologyNational Taipei University of TechnologyTaipei 106 Taiwan;</p> <p>Biomedical Platform and Incubation Services DivisionInstrument Technology Research Center National Applied Research LaboratoriesHsinchu 300 Taiwan;</p> <p>Department of Chemical Engineering and BiotechnologyNational Taipei University of TechnologyTaipei 106 Taiwan;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=DNA intercalator&option=203" rel="nofollow">DNA intercalator;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Biosensor&option=203" rel="nofollow">Biosensor;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Loop-mediated isothermal amplification (LAMP)&option=203" rel="nofollow">Loop-mediated isothermal amplification (LAMP);</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Real-time electrochemistry&option=203" rel="nofollow">Real-time electrochemistry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Food detection&option=203" rel="nofollow">Food detection;</a> </p> <div class="translation"> 机译：DNA嵌入剂;生物传感器;环介导的等温扩增（灯）;实时电化学;食物检测; 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