The expression levels of interleukin-6 (IL-6), IL-12, and matrix metalloproteinase-13 (MMP-13) in the synovial fluid and synovium in hip osteoarthritis and their relationship with disease progression

摘要

Objective: To investigate the expressions of interleukin-6 (IL-6), interleukin-12 (IL-12), and matrix metalloproteinase-13 (M MP-13) in the synovial fluid and synovium in hip osteoarthritis and their relationship with disease progression. Methods: Seventy-two patients with hip osteoarthritis (study group, age 50-80 years) and 43 patients with total hip replacement caused by trauma (control group, age 40-78 years) who were admitted to our hospital from March 2012 to March 2014 were included in our survey. The levels of IL-6, IL-12, and MMP-13 were determined by ELISA; the correlations between IL-6, IL-12, MMP-13 and the Kellgren-Lawrence (K-L) classification were tested using a Spearman analysis; the correlations between IL-6, IL-12, MMP-13 were tested using a Pearson analysis; the critical levels and application values of IL-6, IL-12, and M MP-13 in the K-L classification were also determined using an ROC curve. Results: The levels of IL-6, IL-12, and MMP-13 in the synovial fluid of the study group were higher than those of the control group (all P<0.05). The results of the analysis among the patients with different K-L grades showed that there were significant differences in the levels of IL-6, IL-12, and MMP-13 in the study group. With the increase in the K-L grades, the levels of IL-6, IL-12, and MMP-13 increased (all P<0.05). A Spearman correlation analysis showed that IL-6 (r=0.771, P<0.001), IL-12 (r=0.463, P<0.001), and MMP-13 (r=0.625, P<0.001) were positively correlated the with K-L classification. The Pearson correlation analysis showed that IL-6 was positively correlated with IL-12 (r=0.346, P=0.003), MMP-13 (r=0.518, P<0.001), and IL-12 was positively correlated with MMP-13 (r=0.536, P<0.001). The critical values of IL-6, IL-12 and MMP-13 for the differentiating K-L classification I from II were 144.00 pg/mL, 143.00 pg/mL, 226.80 ng/mL, and the area under the curve (AUC) values were 0.995, 0.645, and 0.785 respectively; for the K-L classifications II to III, the values were 184.70 pg/mL, 193.40 pg/mL and 264.60 ng/mL, and the AUC values were 0.555, 0.760, and 0.740, respectively. Conclusion: The expression levels of IL-6, IL-12, and MMP-13 in the synovial fluid of patients with hip osteoarthritis increased with the severity of the osteoarthritis. In addition, IL-6, IL-12, and MMP-13 had a good correlation with the K-L classification and had a good application value in evaluating the severity of osteoarthritis.