Family of Plasmid Vectors for the Expression of beta-galactosidase Fusion Proteins in Eukaryotic Cells

摘要

LacZ is a popular reporter gene for a variety of biochemical and genetic analyses. The enzymatic activity of its protein product, beta-galactosidase, can be easily measured by well-developed spectrophotometric (4) and luminomet-ric (1) assays in vitro and by histo-chemical staining in vivo (10). Moreover, it is possible to follow the spatial distribution of the gene product in situ, even in living animals, using lipophilic substrates (7,11).