Comparison of serum protein electrophoretic pattern in cows and small ruminants

摘要

Determination of the physiological electrophoretic patterns in animals is very useful for clinicians in diagnosing healthy and sick animals. The objective of this study was to investigate the serum protein electrophoretic pattern in cows, sheep, and goats in order to evaluate the differences in the size and number of protein fractions between the evaluated ruminant species. Ten adult multiparous high-yielding dairy cows, 10 adult female sheep and 10 adult female goats were included in this study. All the evaluated animals were clinically healthy. Serum was analyzed for total serum protein concentrations, and for the relative and absolute values of protein fractions with calculation of albumin/globulin ratios. Serum protein fractions were separated by zone electrophoresis on buffered agarose gel. Serum protein electrophoresis identified 6 distinct bands, comprising albumin, alphai(1)- (alpha(1)), alpha(2)- (alpha(2)), beta(1)- (beta(1)), beta(2)- (beta(2)), and gamma- (gamma) globulins in cows. In sheep, serum proteins exhibited 6 fractions: albumin, alpha(1)-, alpha(2)-, beta-, gamma(1)- and gamma(2)-globulins. In goats, serum proteins were separated into 5 fractions: albumin, alpha(1)-, alpha(2)- beta- and gamma-globulins. Significant differences in the relative as well as absolute means were found for the albumin/globulin ratio and most of the protein fractions, except gamma-globulins. No significant differences were found in the concentration of total proteins. These results describe the marked species differences in most of serum protein fractions between the evaluated groups of animals, and contribute to the current knowledge about the physiological electrophoretic pattern of serum proteins in ruminants, which can be used for diagnostic purposes.