Cellulomonas macrotermitis sp nov., a chitinolytic and cellulolytic bacterium isolated from the hindgut of a fungus-growing termite

摘要

To investigate the symbiotic roles of the gut microbiota in the fungus-growing termite Macrotermes barneyi, a novel strain with chitinolytic and cellulolytic activity, designated strain an-chi-1(T), was isolated from the hindgut of M. barneyi. Strain an-chi-1(T) grows optimally at 28-30 A degrees C, pH 8.0 in PYG medium. On the basis of 16S rRNA gene sequence analysis, this isolate belongs to the genus Cellulomonas with high sequence similarity to Cellulomonas iranensis (99.4%), followed by Cellulomonas flavigena (98.4%), Cellulomonas phragmiteti (97.4%), Cellulomonas oligotrophica (97.2%) and Cellulomonas terrae (97.0%). The DNA-DNA relatedness between an-chi-1(T) and the type strains of C. iranensis and C. flavigena DSM20109(T) are 35.4% and 23.7%, respectively. The major cellular fatty acids are anteiso-C-15:0 and C-14:0. The polar lipid profile consists of diphosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylinositol dimannosides and one unidentified phospholipid. The cell-wall sugar is ribose. The peptidoglycan contains glutamic acid, aspartic acid and alanine. The DNA G+C content is 67.3 mol%. Based on its distinctive phenotypic, phylogenetic, and chemotaxonomic characteristics, an-chi-1(T) represents a novel species of the genus Cellulomonas, for which the name Cellulomonas macrotermitis sp. nov. is proposed. The type strain is an-chi-1(T) (= JCM 31923(T) = CICC 24195(T)).