Extraction and Analysis of Flaxseed Oil by Supercritical CO2 Method Optimized by RSM and GC-MS

摘要

Flaxseed oil has already been widely used in food production in recent years as of its prominent biological functions. This study was aimed to obtain high-quality flaxseed oil and find the suitable extraction parameters, exploiting response surface methodology of a three-variable, three-level Box-Behnken experimental design. In this study, supercritical CO2 extraction technology was used to extract flaxseed oil. The chemical composition of flaxseed oil was investigated by Gas Chromatography-Mass Spectroscopy. Results showed that the optimal conditions were extraction time of 4.58 h, extraction temperature at 51 degrees C, and extraction pressure at 31 MPa. Under these conditions, the highest extraction yield of flaxseed oil was 23.95%. 9, 12, 15-Octadecatrienoic acid was indicated to be the highest content according to GC-MS analysis, with the content of 75.22%. In conclusion, supercritical CO2 extraction was proved to be an effective technique for extracting oil from flaxseed, and Gas Chromatography-Mass Spectroscopy provided the satisfactory method of analyzing flaxseed oil from flaxseed for industrial need.