Genetic diversity in traditional and modern allelopathic rice accessions revealed by minisatellite markers

摘要

This study aimed to assess the genetic diversity among the 46-allelopathic rice accessions from Bangladesh Rice Research Institute (BRRI) and Malaysian Agricultural Research and Development Institute (MARDI) and Africa using 20 minisatellite or inter simple sequence repeat (ISSR) markers. Out of 20 ISSR markers, 10-markers generated 188 polymorphic loci. The highest polymorphic loci (41) were found in accession BRRI dhan47, whereas, the lowest was in BR11 (22). Mean Shannon's information index and polymorphic information content were 0.37 and 0.19, respectively, in ten markers. The mean effective number of alleles in the 10-ISSR markers varied from 1.24 to 1.56. The number of polymorphic loci was highest (29) in WITA3 and lowest (24) in WITA12 accessions of African population. The cluster analysis revealed 10-main groups, including several subgroups at 0.31 similarity coefficient. The principal component analysis elucidated 36.94% complete variations in all accessions in this study. There was considerable genetic diversity in rice accessions in this study. The information about genetic diversity and relationship obtained from the study may be used to develop the allelopathic rice in future breeding programmes.