摘要

Control of pests in soybean farmers commonly use a chemical synthetic pesticide that has a negative impact both on people and the agroecosystem. Another biological control methods are effective and safe against agroecosystem is the use of microorganisms such as viruses Spodopteralitura Multiple Nucleopolyhedrosis Virus ( Splt MNPV), fungus ( Beauveria bassiana ), Neem Seed ( Azadirachta indica ), they are not resistance to the solar radiation. The study aim was to formulate the combination of solar radiation-resistant bioinsecticides that have abilityagainst Spodoptera litura , Nezara viridulla and Riptortus (non-target pest) and Menochilus sexmaculatus (major pest predator). Splt MNPV was multiplied in the epithelial cell culture. Neem seed was extracted and B. bassiana was isolated for biopesticidesamples. The various combination of biopesticide used to treat pest (target and non-target organisms) in laboratory and greenhouse. The mortality was observed for 17 days. The morphological and histological analysis was conducted to evaluate the effect of biopesticide formulation. The resultsshowed that formulation of photoprotectant and vegetable microbial biopesticide was Splt MNPV + B. bassiana +NSE+Kaolin+EPMS 15% (1:1:1:4:15%) resulted in 93.5% mortality. This biopesticide formula was compatible to control target pest indicated by morphological and histologicalfeatures by the presence of three symptoms in reaction with active ingredients, those were dead with aqueous body, followed by fungal hyphae growth, hardened body, the presence of PIBs virus and fungal hyphae as seen from histological images, as well as reduced epithelia in some cells.