SILDENAFIL UPREGULATES TUMOR NECROSIS FACTOR alpha PRODUCTION IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF HEALTHY MEN - PRELIMINARY REPORT

摘要

Sildenafil is a selective type 5 phosphodiesterase (PDE5) inhibitor, commonly used in the treatment of erectile dysfunction (ED) and pulmonary arterial hypertension (PAH). The results of recent studies suggest that this drug modulates function of the immune system. However, only few studies were performed in humans. Therefore, the aim of this study was to investigate the ability of sildenafil to regulate the production of proinflammatory cytokines including tumor necrosis factor alpha (TNF-alpha), interleukin (1L)-1 beta and IL-6 in peripheral blood mononuclear cells (PBMCs) from healthy men. Phorbol myristate acetate (PMA) - stimulated and unstimulated PBMCs from 16 participants were cultured in the absence or presence of sildenafil (400 ng/mL and 4 pg/mL). TNF-alpha, IL-1 beta and IL-6 concentrations in culture supernatants were measured by enzyme-linked immunosorbent assay (ELISA). Cell viability was assessed by trypan blue staining. Sildenafil at a concentration of 400 ng/mL significantly increased TNF-alpha. production in stimulated PBMCs (p < 0.05) hut had no effects on 1L-1 beta and 1L-6. The drug did not affect PBMCs viability. This is the first report describing such effects of sildenafil in humans.