HTLC: A Viable Approach to Fast LC of Biogenic Amines in Food with Legacy Equipment

摘要

Nowadays, there is a demand for fast liquid chromatography (LC) of biogenic amines in food with the equipment available in the average laboratory. To this end, high-temperature liquid chromatography (HTLC) is presented in this study as a viable option, working on a conventional LC platform at moderately high temperatures up to 80?°C. The LC platform was adapted by including an appropriate length of stainless-steel microbore tubing as a preheater. Biogenic amines as benzoyl derivatives showed good thermostability on a thermally rugged column (150?×?4.6?mm, 5?μm). As a model application the HTLC conditions were developed for wine separation. The separation selectivity changed considerably with temperature in the range 36–84?°C, and baseline resolution was obtained only at temperatures well above ambient. At 73?°C oven temperature, the system allowed a flow rate as high as 3?mL?min_(?1)with the backpressure not?exceeding 195?bar (2800?psi). The separation took less than 5?min, comparably fast to documented fast LC separations, and typically at least three to five times faster than a conventional separation.