Template‐Synthesized Vertical Needle Array as Injection Platform for Microalgae

Mukaibo Hitomi

摘要

Abstract > Increasing numbers of studies in the past few decades have demonstrated vertically‐oriented nanoneedles arrays (NNAs) as innovative tools to interrogate and manipulate biological cells, where the needles are inserted into the cells as functional probes for high‐throughput detection and biomolecule delivery. However, majority of these studies use mammalian cells: leaving NNA application to plant cells still in its infancy and largely unexplored. This paper highlights our contributions in exploring the utility of NNAs to microalgae – a diverse group of aquatic, photosynthetic organisms studied intensively as bio‐factories for producing high‐value‐added products such as fuels and pharmaceuticals. Microalgal strain development processes have long suffered from the hard cell wall that surrounds the cell and inhibits delivery of foreign materials into the cell. Conically‐shaped, metallic NNAs were developed with template synthesis that successfully penetrate through the cell wall barrier and achieve material injection – using the widely studied model microalga, Chlamydomonas reinhardtii . Earlier works from mammalian cells are introduced and discussed to clarify the framework established in this field, while recent studies of both mammalian and microalgal cells are also referenced to examine the trends, challenges, and future perspectives of NNA application to microalgae. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >过去几十年的研究数量越来越多地显示了垂直导向的纳尼德阵列（NNA）作为创新工具来询问和操纵生物细胞，其中针被插入细胞中作为用于高通量检测和生物分子递送的功能探针。然而，这些研究中的大多数使用哺乳动物细胞：将NNA申请留在植物细胞中，仍然在其婴儿期并且在很大程度上未开发。本文突出了我们在探索NNA对微藻的效用 - 一种多样化的水生，光合生物，与生物工厂进行了集中研究，以生产高附加值产品，如燃料和药品。微藻菌株发育过程长期患有围绕细胞的硬细胞壁，并抑制异物递送到细胞中。圆锥形的金属NNA具有模板合成，成功穿过细胞壁屏障并实现材料注射 - 使用广泛研究的模型微藻，衣原体Reinhardtii </ i>。介绍哺乳动物细胞的早期作品被介绍并讨论以澄清该领域中建立的框架，而最近的哺乳动物和微藻细胞的研究也被称为审查NNA应用对微藻的趋势，挑战和未来的观点。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16132/'>《Chemical record》</a> <b style="margin: 0 2px;">|</b><span>2019年第5期</span><b style="margin: 0 2px;">|</b><span>共14页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mukaibo Hitomi&option=202" target="_blank" rel="nofollow">Mukaibo Hitomi;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Chemical EngineeringUniversity of Rochester 4510 Wegmans Hall Rochester NY14627 USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Electrodeposition&option=203" rel="nofollow">Electrodeposition;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Injection&option=203" rel="nofollow">Injection;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Microalgae&option=203" rel="nofollow">Microalgae;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nanostructures&option=203" rel="nofollow">Nanostructures;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Needle array&option=203" rel="nofollow">Needle array;</a> </p> <div class="translation"> 机译：电沉积;注射;微藻;纳米结构;针阵列; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022080056.html">Template‐Synthesized Vertical Needle Array as Injection Platform for Microalgae</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mukaibo Hitomi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Mukaibo Hitomi </a> <a href="/journal-foreign-16132/" target="_blank" rel="nofollow" class="tuijian_authcolor">Chemical record .</a> <span>2019</span><span>,第5期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：模板合成的垂直针阵作为微藻喷射平台</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704015048513.html">Are needle-free injections a useful alternative for growth hormone therapy in children? 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