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首页> 外文期刊>Cellular microbiology >A proteomic analysis unravels novel CORVET and HOPS proteins involved in Toxoplasma gondiiToxoplasma gondii secretory organelles biogenesis
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A proteomic analysis unravels novel CORVET and HOPS proteins involved in Toxoplasma gondiiToxoplasma gondii secretory organelles biogenesis

机译：蛋白质组学分析揭开新型肉豆蔻和啤酒花蛋白涉及弓形虫弓形虫弓形虫分泌细胞器生物发生

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Abstract > Apicomplexans use the endolysosomal system for the biogenesis of their secretory organelles, namely, micronemes, rhoptries, and dense granules. In Toxoplasma gondii , our previous in silico search identified the HOPS tethering but not the CORVET complex and demonstrated a role of Vps11 (a common component for both complexes) in its secretory organelle biogenesis. Herein, we performed Vps11‐GFP‐Trap pull‐down assays and identified by proteomic analysis, not only the CORVET‐specific subunit Vps8 but also a BEACH domain‐containing protein (BDCP) conserved in eukaryotes. We show that knocking‐down Vps8 affects targeting of dense granule proteins, transport of rhoptry proteins, and the localization of the cathepsin L protease vacuolar compartment marker. Only a subset of micronemal proteins are affected by the absence of Vps8, shedding light on at least two trafficking pathways involved in microneme maturation. Knocking‐down BDCP revealed a restricted and particular role of this protein in rhoptry and vacuolar compartment biogenesis. Moreover, depletion of BDCP or Vps8 abolishes parasite virulence in vivo. This study identified BDCP as a novel CORVET/HOPS‐associated protein, playing specific roles and acting in concert during secretory organelle biogenesis, an essential process for host cell infection. Our results open the hypothesis for a role of BDCP in the vesicular trafficking towards lysosome‐related organelles in mammals and yeast. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”> Abstract </ title> > Apicomplex人使用indolysoomal系统进行其分泌细胞器的生物发生，即微米，毒液，革命，和致密的颗粒。在毒素弓形虫</ i>中，我们在硅搜查中的先前确定了啤酒花培养物，但不是慢速复合物，并证明了VPS11（综合组分的共同组分）在其分泌细胞器生物发生中的作用。在此，我们通过蛋白质组学分析进行了VPS11-GFP-TRAP下拉测定并鉴定，不仅是甲状腺特异性亚基VPS8，而且鉴定了含沙地结构域的蛋白质（BDCP）。我们表明爆震VPS8会影响致密颗粒蛋白，鼻腔蛋白的运输，以及组织蛋白酶L蛋白酶真空室标记物的定位。只有vps8的缺失影响了微量蛋白质的子集，脱落在至少两种贩运途径上涉及微量肿瘤成熟的途径。爆震BDCP揭示了该蛋白质在鼻腔和真空室生物发生中的限制性和特殊作用。此外，BDCP或VPS8的耗尽废除了体内寄生虫毒力。本研究将BDCP作为一种新型磁性/蛇麻碱相关蛋白，在分泌细胞器生物发生期间在分泌细胞器生物发生期间参加特异性作用，并在音乐会中作用，是宿主细胞感染的必要过程。我们的结果开启了BDCP在哺乳动物和酵母中对溶酶体相关的细胞器的粘质贩运的作用的假设。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15891/'>《Cellular microbiology》</a> <b style="margin: 0 2px;">|</b><span>2018年第11期</span><b style="margin: 0 2px;">|</b><span>共19页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Morlon‐Guyot Juliette&option=202" target="_blank" rel="nofollow">Morlon‐Guyot Juliette;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=El Hajj Hiba&option=202" target="_blank" rel="nofollow">El Hajj Hiba;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Martin Kevin&option=202" target="_blank" rel="nofollow">Martin Kevin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fois Adrien&option=202" target="_blank" rel="nofollow">Fois Adrien;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Carrillo Amandine&option=202" target="_blank" rel="nofollow">Carrillo Amandine;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Berry Laurence&option=202" target="_blank" rel="nofollow">Berry Laurence;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Burchmore Richard&option=202" target="_blank" rel="nofollow">Burchmore Richard;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Meissner Markus&option=202" target="_blank" rel="nofollow">Meissner Markus;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lebrun Maryse&option=202" target="_blank" rel="nofollow">Lebrun Maryse;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Daher Wassim&option=202" target="_blank" rel="nofollow">Daher Wassim;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Dynamique des Interactions Membranaires Normales et Pathologiques UMR5235 CNRS INSERMUniversité de MontpellierMontpellier France;</p> <p>Departments of Internal Medicine and Experimental Pathology Immunology and MicrobiologyAmerican University of BeirutBeirut Lebanon;</p> <p>Dynamique des Interactions Membranaires Normales et Pathologiques UMR5235 CNRS INSERMUniversité de MontpellierMontpellier France;</p> <p>Dynamique des Interactions Membranaires Normales et Pathologiques UMR5235 CNRS INSERMUniversité de MontpellierMontpellier France;</p> <p>Dynamique des Interactions Membranaires Normales et Pathologiques UMR5235 CNRS INSERMUniversité de MontpellierMontpellier France;</p> <p>Dynamique des Interactions Membranaires Normales et Pathologiques UMR5235 CNRS INSERMUniversité de MontpellierMontpellier France;</p> <p>Polyomics FacilityUniversity of GlasgowGlasgow UK;</p> <p>Wellcome Centre for Molecular ParasitologyUniversity of GlasgowGlasgow UK;</p> <p>Dynamique des Interactions Membranaires Normales et Pathologiques UMR5235 CNRS INSERMUniversité de MontpellierMontpellier France;</p> <p>Dynamique des Interactions Membranaires Normales et Pathologiques UMR5235 CNRS INSERMUniversité de MontpellierMontpellier France;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/173.html" title="普通生物学">普通生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=anterograde trafficking&option=203" rel="nofollow">anterograde trafficking;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Apicomplexa&option=203" rel="nofollow">Apicomplexa;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=BEACH domain‐containing protein&option=203" rel="nofollow">BEACH domain‐containing protein;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=CORVET&option=203" rel="nofollow">CORVET;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=HOPS&option=203" rel="nofollow">HOPS;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=invasion&option=203" rel="nofollow">invasion;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=membranous fusion and fission events&option=203" rel="nofollow">membranous fusion and fission events;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=secretory organelles&option=203" rel="nofollow">secretory organelles;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tet‐inducible system&option=203" rel="nofollow">Tet‐inducible system;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Toxoplasma gondii&option=203" rel="nofollow">Toxoplasma gondii;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Vps8&option=203" rel="nofollow">Vps8;</a> </p> <div class="translation"> 机译：Anterograde贩运;Apicomplexa;含沙滩域的蛋白质;慢跑;蛇;入侵;膜融合和裂变事件;分泌细胞器;TET-INGUIBLE SYSTEM;Toxoplasma Gondii;vps8; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022031945.html">A proteomic analysis unravels novel CORVET and HOPS proteins involved in <fi >Toxoplasma gondii</fi>Toxoplasma gondii secretory organelles biogenesis</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Morlon‐Guyot Juliette&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Morlon‐Guyot Juliette,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=El Hajj Hiba&option=202" target="_blank" rel="nofollow" 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Oesterlin,</a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：弓形虫囊泡蛋白分选VPS9在分泌细胞器生物发生和宿主感染中的关键作用。</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000132257389.html">Toxoplasma gondii Vps11, a subunit of HOPS and CORVET tethering complexes, is essential for the biogenesis of secretory organelles</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Juliette Morlon‐Guyot&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Juliette Morlon‐Guyot,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sandra Pastore&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sandra Pastore,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Laurence Berry&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Laurence Berry,</a> <span>2015</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：弓形虫Gondii vps11，啤酒花和肉瘤束缚复合物的亚基对分泌细胞器的生物发生至关重要</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201292851885.html">应用生物素标记与蛋白质组学方法分离鉴定弓形虫表面蛋白和分泌蛋白</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘媛&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘媛</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=薛峰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,薛峰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄敏君&option=202" target="_blank" rel="nofollow" class="tuijian_auth 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2013</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_china-health-care-nutrition_thesis/0201275106555.html">可溶性弓形虫棒状体分泌蛋白2(ROP2)的免疫原性和抗弓形虫感染核酸疫苗的研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈晨&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈晨</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=公茂庆&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,公茂庆</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=寇景轩&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,寇景轩</a> <span> <a href="/journal-cn-5432/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国保健营养(上旬刊) </a> </span> <span> . 2013</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_gansu-animal-husbandry-veterinary_thesis/0201293346687.html">弓形虫重要药物靶细胞器蛋白转运机制被揭示</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/journal-cn-7074/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 甘肃畜牧兽医 </a> </span> <span> . 2021</span><span>,第9期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-tropical-diseases-parasitology_thesis/0201230954857.html">弓形虫微线体分泌蛋白TgMIC10基因的克隆和序列分析</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张松涛&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 张松涛</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=汪学龙&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,汪学龙</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=沈继龙&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,沈继龙</a> <span> <a href="/journal-cn-5974/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 热带病与寄生虫学 </a> </span> <span> . 2006</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-5670_thesis/020221419146.html">刚地弓形虫尿苷磷酸化酶基因编码蛋白的生物信息学分析、蛋白重组及免疫保护性</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=祝建疆&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 祝建疆</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李雅清&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李雅清</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王海龙&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王海龙</a> <span> <a href="/conference-cn-5670/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第五届北京热带医学与寄生虫学论坛 </a> <span> <span> . 2013</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315279084.html">弓形虫感染宿主细胞磷酸化蛋白质组学分析及宿主细胞蛋白vimentin在弓形虫感染过程中的作用机制研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=何成&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 何成</a> <span> . 2018</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061204382970.html">一种弓形虫棒状体蛋白ROP18的单克隆抗体、分泌该抗体的细胞株及其用途</a> <b>[P]</b> . <span> 中国专利： CN109082412B </span> <span> . 2021.05.04</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120111584308.html">一种弓形虫棒状体蛋白ROP18的单克隆抗体、分泌该抗体的细胞株及其用途</a> <b>[P]</b> . <span> 中国专利： CN109082412A </span> <span> . 2018-12-25</span> 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break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：弓形虫特异的遗传问题及其在弓形虫体外检测和分型中的应用 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130411558204.html">COMPOSITION AND KIT FOR DIAGNOSING ACUTE AND CHRONIC INFECTION OF TOXOPLASMA GONDII, AND METHOD FOR DIAGNOSING ACUTE AND CHRONIC INFECTION OF TOXOPLASMA GONDII</a> <b>[P]</b> . <span> 外国专利： <!-- 韩国专利： --> KR101581036B1 </span> <span> . 2015-12-29</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：诊断弓形虫急性和慢性感染的组合物和试剂盒，以及诊断弓形虫的急性和慢性感染的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 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