Oxygen and contact with human intestinal epithelium independently stimulate virulence gene expression in enteroaggregative        <fc >          <fi>Escherichia coli</fi>        </fc>          <fi >Escherichia coli</fi>Escherichia coli

Ellis Samuel J.; Yasir Muhammad; Browning Douglas F.; Busby Stephen J.W.; Schüller Stephanie

摘要

Abstract > Enteroaggregative <fc> <fi>Escherichia coli</fi> </fc> (EAEC) are important intestinal pathogens causing acute and persistent diarrhoeal illness worldwide. Although many putative EAEC virulence factors have been identified, their association with pathogenesis remains unclear. As environmental cues can modulate bacterial virulence, we investigated the effect of oxygen and human intestinal epithelium on EAEC virulence gene expression to determine the involvement of respective gene products in intestinal colonisation and pathogenesis. Using in vitro organ culture of human intestinal biopsies, we established the colonic epithelium as the major colonisation site of EAEC strains 042 and 17‐2. We subsequently optimised a vertical diffusion chamber system with polarised T84 colon carcinoma cells for EAEC infection and showed that oxygen induced expression of the global regulator AggR, aggregative adherence fimbriae, <fc> <fi>E.?coli</fi> </fc> common pilus, EAST‐1 toxin, and dispersin in EAEC strain 042 but not in 17‐2. Furthermore, the presence of T84 epithelia stimulated additional expression of the mucinase Pic and the toxins HlyE and Pet. This induction was dependent on physical host cell contact and did not require AggR. Overall, these findings suggest that EAEC virulence in the human gut is modulated by environmental signals including oxygen and the intestinal epithelium. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：lang =“en”> <标题类型=“main”>抽象</ title> > enderoaggregative <fc> <fi> escherichia coli </ fi> </ fc>（eaec ）重要的肠道病原体导致全世界急性和持续的腹泻病。虽然已经鉴定了许多推定的EAEC毒力因子，但它们与发病机制的关联仍不清楚。随着环境提示可以调节细菌毒力，我们研究了氧气和人肠上皮对EAEC毒力基因表达的影响，以确定各种基因产物在肠道定植和发病机制中的参与。使用人类肠道活检的体外器官培养，我们将结肠上皮作为EAEC菌株042和17-2的主要定植位点。我们随后优化了具有偏振T84结肠癌细胞的垂直扩散室系统，用于EAEC感染，并表明氧气诱导全球调节剂胚胎，聚合粘附性FIMBRIAE，<FC> <FI> E.?COLI </ FI> </ FC的表达>普通菌落，东1毒素，和分散素在EAEC菌株042，但不是17-2。此外，T84上皮的存在刺激了粘蛋白PIC和毒素Hlye和PET的另外表达。这种诱导依赖于物理宿主细胞接触，并且不需要agag。总体而言，这些发现表明人体肠道中的EAEC毒力由包括氧和肠上皮的环境信号调节。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15891/'>《Cellular microbiology》</a> <b style="margin: 0 2px;">|</b><span>2019年第6期</span><b style="margin: 0 2px;">|</b><span>共12页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ellis Samuel J.&option=202" target="_blank" rel="nofollow">Ellis Samuel J.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yasir Muhammad&option=202" target="_blank" rel="nofollow">Yasir Muhammad;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Browning Douglas F.&option=202" target="_blank" rel="nofollow">Browning Douglas F.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Busby Stephen J.W.&option=202" target="_blank" rel="nofollow">Busby Stephen J.W.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Schüller Stephanie&option=202" target="_blank" rel="nofollow">Schüller Stephanie;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Norwich Medical SchoolUniversity of East AngliaNorwich UK;</p> <p>Quadram Institute BioscienceNorwich UK;</p> <p>Institute of Microbiology and InfectionUniversity of BirminghamBirmingham UK;</p> <p>Institute of Microbiology and InfectionUniversity of BirminghamBirmingham UK;</p> <p>Norwich Medical SchoolUniversity of East AngliaNorwich UK;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/173.html" title="普通生物学">普通生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=adherence&option=203" rel="nofollow">adherence;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=colonic epithelium&option=203" rel="nofollow">colonic epithelium;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=diarrhoea&option=203" rel="nofollow">diarrhoea;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=EAEC&option=203" rel="nofollow">EAEC;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=gene expression&option=203" rel="nofollow">gene expression;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=oxygen&option=203" rel="nofollow">oxygen;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=virulence&option=203" rel="nofollow">virulence;</a> </p> <div class="translation"> 机译：依恋;结肠上皮;腹泻;EAEC;基因表达;氧气;毒力; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" 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2013</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_oceanologia-limnologia-sinica_thesis/0201253921685.html">乳糖诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichia coli)中的表达</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=唐志红&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 唐志红</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=葛保胜&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,葛保胜</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=林凡&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,林凡</a> <span> <a href="/journal-cn-4981/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 海洋与湖沼 </a> </span> <span> . 2006</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-8429_thesis/020222139747.html">猪肠外大肠杆菌毒力基因检测及耐药性研究</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=曾博&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 曾博</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王红宁&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王红宁</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐昌文&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,徐昌文</a> <span> <a href="/conference-cn-8429/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 四川省畜牧兽医学会2012年学术年会 </a> <span> <span> . 2012</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020316368875.html">基于Wi--Fi信号非接触式感知的人体行为识别应用研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王福超&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王福超</a> <span> . 2021</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120113821567.html">基于Wi-Fi信道状态信息的无接触式人体行为识别方法及系统</a> <b>[P]</b> . <span> 中国专利： CN113837122A </span> <span> . 2021-12-24</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120111030313.html">一种基于商用Wi-Fi的非接触多人计步方法及系统</a> <b>[P]</b> . <span> 中国专利： CN108548545A </span> <span> . 2018-09-18</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130400497340.html">GENE-THERAPEUTIC DNA-VECTOR BASED ON GENE-THERAPEUTIC DNA-VECTOR VTVAF17, CARRYING TARGET GENE SELECTED FROM GROUP OF GENES ANG, ANGPT1, VEGFA, FGF1, HIF1Α, HGF, SDF1, KLK4, PDGFC, PROK1, PROK2 TO INCREASE EXPRESSION LEVEL OF SAID TARGET GENES, METHOD FOR PRODUCTION AND USE THEREOF, STRAIN ESCHERICHIA COLI SCS110-AF/VTVAF17-ANG, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-ANGPT1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-VEGFA, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-FGF1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-HIF1Α, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-HGF, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-SDF1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-KLK4, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-PDGFC, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-PROK1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-PROK2, CARRYING GENE-THERAPEUTIC DNA VECTOR, METHOD FOR PRODUCTION THEREOF, METHOD FOR INDUSTRIAL PRODUCTION OF GENE-THERAPEUTIC DNA VECTOR</a> <b>[P]</b> . <span> 外国专利：  RU2730664C2 </span> <span> . 2020-08-24</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于基因治疗DNA载体VTVAF17的基因治疗DNA载体，携带选自基因ANG，ANGPT1，VEGFA，FGF1，HIF1α，HGF，SDF1，KLK4，PDGFC，PROK1，PROK2表达的靶基因所述的靶标基因，其生产和使用方法，应变大肠埃希氏菌SCS110-AF / VTVAF17-ANG或大肠埃希氏菌SCS110-AF / VTVAF17-ANGPT1或大肠埃希氏菌SCS110-AF / VTVAF17-VEGFA或大肠埃希氏菌SCS110-AF / VTVAF17-FGF1，或大肠埃希氏菌SCS110-AF / VTVAF17-HIF1A，或大肠埃希氏菌COLI SCS110-AF / VTVAF17-HGF，或大肠埃希氏菌SCS110-AF / VTVAF17-SDF1，或大肠埃希氏菌SCS110-AF / VTVAF17-KLK4，大肠埃希氏菌SCS110-AF / VTVAF17-PDGFC或大肠埃希氏菌SCS110-AF / VTVAF17-PROK2，携带基因-治疗性DNA载体，生产方法，工业生产方法-治疗性DNA载体 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130400537005.html">Gene therapeutic DNA vector based on VTvaf17 gene therapeutic DNA vector carrying a target gene selected from the group of genes ANG, ANGPT1, VEGFA, FGF1, HIF1α, HGF, SDF1, KLK4, PDGFC, PROK1, PROK2 to increase the expression level of these target genes, method its preparation and use, Escherichia coli strain SCS110-AF / VTvaf17-ANG or Escherichia coli SCS110-AF / VTvaf17-ANGPT1 or Escherichia coli SCS110-AF / VTvaf17-VEGFA or Escherichia coli SCS110-AF / VTvaf17-Fichia-SC110 AF / VTvaf17-HIF1α or Escherichia coli SCS110-AF / VTvaf17-HGF or Escherichia coli SCS110-AF / VTvaf17-SDF1 or Escherichia coli SCS110-AF / VTvaf17-KLK4 or Escherichia coli SCS110-AF / VTviFi10 SCF110 AF / VTvaf17-PROK1 or Escherichia coli SCS110-AF / VTvaf17-PROK2 carrying a gene therapy DNA vector, a method for its preparation, a method for the industrial production of a gene therapy DNA vector</a> <b>[P]</b> . <span> 外国专利：  RU2018147085A </span> <span> . 2020-06-29</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于VTvaf17基因治疗DNA载体的基因治疗DNA载体，其携带选自ANG，ANGPT1，VEGFA，FGF1，HIF1α，HGF，SDF1，KLK4，PDGFC，PROK1，PROK2的靶基因以增加这些靶标的表达水平基因，方法的制备和使用，大肠杆菌菌株SCS110-AF / VTvaf17-ANG或大肠杆菌SCS110-AF / VTvaf17-ANGPT1或大肠杆菌SCS110-AF / VTvaf17-VEGFA或大肠杆菌SCS110-AF / VTvaf17-Fichia-SC110 AF /VTvaf17-HIF1α或大肠杆菌SCS110-AF / VTvaf17-HGF或大肠杆菌SCS110-AF / VTvaf17-SDF1或大肠杆菌SCS110-AF / VTvaf17-KLK4或大肠杆菌SCS110-AF / VTviFi10 S1携带基因治疗DNA载体的大肠杆菌SCS110-AF / VTvaf17-PROK2，其制备方法，工业生产基因治疗DNA载体的方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130400493335.html">GENE-THERAPEUTIC DNA VECTOR BASED ON THE GENE-THERAPEUTIC DNA VECTOR GDTT1_8NAS12, CARRYING THE TARGET GENE SELECTED FROM A GROUP OF GENES DDC, IL10, IL13, IFNB1, TNFRSF4, TNFSF10, BCL2, HGF, IL2 TO INCREASE THE EXPRESSION LEVEL OF SAID TARGET GENES, A METHOD FOR PRODUCTION AND USE THEREOF, A STRAIN ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-DDC OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-IL10 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-IL13 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-IFNB1 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-TNFRSF4 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-TNFSF10 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-BCL2 OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-HGF OR ESCHERICHIA COLI JM110-NAS/GDTT1_8NAS12-IL2, CARRYING A GENE-THERAPEUTIC DNA VECTOR, METHOD FOR PRODUCTION THEREOF, A METHOD FOR INDUSTRIAL PRODUCTION OF A GENE-THERAPEUTIC DNA VECTOR</a> <b>[P]</b> . <span> 外国专利：  RU2734726C1 </span> <span> . 2020-10-22</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于基因治疗DNA矢量GDTT1_8NAS12的基因治疗DNA矢量，携带从一组基因中选择的目标基因DDC，IL10，IL13，IFNB1，TNFRSF4，TNFSF10，BCL2，HGF，IL2可以增加表达水平基因，一种生产和使用其的方法，应变大肠埃希氏菌JM110-NAS / GDTT1_8NAS12-DDC或大肠埃希氏菌JM110-NAS / GDTT1_8NAS12-IL10或大肠埃希氏菌JM110-NAS / GDTT1_8NAS12-IL13或大肠埃希氏菌COLI JM110-NAS / GDTT1_8NAS12-IL13 IFNB1或ESCHERICHIA COLI JM110-NAS / GDTT1_8NAS12-TNFRSF4或ESCHERICHIA COLI JM110-NAS / GDTT1_8NAS12-TNFSF10或ESCHERICHIA COLI JM110-NAS / GDTT1_8NAS12-BCL2或ESCHERICHIA COLI JM110-NAS / GDTT1_8NAS12 IL2，携带基因治疗性DNA载体，其生产方法，工业生产基因治疗性DNA载体的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> 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Oxygen and contact with human intestinal epithelium independently stimulate virulence gene expression in enteroaggregative Escherichia coli Escherichia coliEscherichia coli

摘要