Bovine peripheral blood WC1(+) and WC1(neg) gamma delta T lymphocytes modulate monocyte-derived macrophage effector functions during in vitro Mycobacterium avium subspecies paratuberculosis infection

摘要

The importance of bovine gamma delta T lymphocytes during anti-mycobacterial immunity is recognized; however, the role of major subsets of gamma delta T lymphocytes (WC1(+) and WC1(neg)) in this process remains unclear. We investigated how WC1(+) and WC1(neg) gamma delta T lymphocyte subsets of calves modulate monocyte-derived macrophage (MDM) functions during Map infection in vitro. To achieve this, Map-infected or uninfected MDMs from young calves were co-cultured with autologous WC1(+) or WC1(neg) gamma delta T lymphocytes. Our data indicate that WC1(+) and WC1(neg) gamma delta T lymphocytes of young calves modulate effector functions of MDMs with respect to Map killing, CD11b and MHC-II expression. We observed differences in IFN-gamma production and CD25 expression on gamma delta T lymphocyte subsets, as well as MDM expression of CD1b when in contact with WC1(neg) gamma delta T lymphocytes. (C) 2017 Elsevier Inc. All rights reserved.