Determination of Chemical and Enantiomeric Purity of α-Amino Acids and their Methyl Esters as N-FIuorenylmethoxycarbonyl Derivatives Using Amyiose-derived Chiral Stationary Phases

摘要

Liquid chromatographic enantiomer separation and simultaneous determination of chemical and enantiomeric purity of a-amino acids and their methyl esters as N-fluorenylmethoxycarbonyl (FMOC) derivatives was performed on three covalently bonded type chiral stationary phases (CSPs) derived from amylose derivatives. The enantiomer separation of a-amino acid esters as N-FMOC derivatives was better than that of the corresponding acids, especially for CSP 1 and 2. Chemical impurities as the corresponding racemic acids present in several commercially available racemic amino acid methyl esters were observed to be 0.49-17.50%. Enantiomeric impurities of several commercially available L-amino acid methyl esters were found to be 0.03-0.58%, whereas chemical impurities as the corresponding racemic acids present in the same analytes were found to be 0.13-13.62%. This developed analytical method will be useful for the determination of chemical and enantiomeric purity of α-amino acids and/or esters as N-FMOC derivatives using amyiose-derived CSPs.