Large‐scale purification of high purity α1‐antitrypsin from Cohn Fraction IV with virus inactivation by solvent/detergent and dry‐heat treatment

Huangfu Chaoji; Zhang Jinchao; Ma Yuyuan; Jia Junting; Li Jingxuan; Lv Maomin; Ma Xiaowei; Zhao Xiong; Zhang Jingang

摘要

Abstract > α1‐Antitrypsin (AAT) is widely used to treat patients with congenital AAT deficiency. Cohn Fraction IV (Cohn F IV) is normally discarded during the manufacturing process of albumin but contains approximately 33% of plasma AAT. We established a new process for large‐scale purification of AAT from it. liquid chromatography‐electrospray ionization‐tandem mass spectrometry and high‐performance liquid chromatography were applied for qualitative identification and composition analysis, respectively. Stabilizers were optimized for AAT activity protection during lyophilization and dry‐heat. Virus inactivation by dry‐heat and solvent/detergent (S/D) was validated on a range of viruses. AAT with purity of 95.54%, specific activity of 3,938.5?IU/mg, and yield of 26.79%, was achieved. More than 95% activity was reserved after S/D. More than 96% activity was obtained after lyophilization or dry‐heat. After S/D, pseudorabies virus (PRV) and vesicular stomatitis virus (VSV) were inactivated below detectable level within 1?H. Virus titer reductions of more than 5.50 log <sub>10</sub> and 5.38 log <sub>10</sub> were achieved for PRV and VSV, respectively. Porcine parvovirus and encephalomyocarditis virus were inactivated by 3.17 log <sub>10</sub> and 5.88 log <sub>10</sub> reduction after dry‐heat. The advantages of this process, including suitability for large‐scale production, high purity, better utilization of human plasma, viral safety, commercial and inexpensive chromatography medium, may facilitate its further application. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >α1-抗真菌（AAT）广泛用于治疗先天性AAT缺乏的患者。在白蛋白的制造过程中通常丢弃COHN馏分IV（COHN F IV），但含有约33％的血浆AAT。我们建立了一种新的进程，可以从中享受大规模净化AAT。液相色谱 - 电喷雾电离 - 串联质谱和高效液相色谱分别用于定性鉴定和成分分析。在冻干和干热期间针对AAT活性保护进行了优化稳定剂。通过干热和溶剂/洗涤剂（S / D）的病毒失活在一系列病毒上验证。 AAT纯度为95.54％，特异性活性为3,938.5？Iu / mg，达到26.79％的产量。 S / D后保留了95％以上的活动。在冻干或干热后获得超过96％的活性。在S / D中，假毒性病毒（PRV）和脉体口腔炎病毒（VSV）在1μl℃的下降水平以下灭活。对于PRV和VSV，分别为PRV和VSV达到了5.50个log <sub> 10 </ sub> 10 </ sub>的病毒滴度。猪剖视病毒和脑膜炎炎病毒通过3.17 log <sub> 10 </ sub>和5.88 log <sub> 10 </ sub>减少在干热后。该方法的优点包括适用于大规模生产，高纯度，更好地利用人血浆，病毒安全性，商业和廉价的色谱介质，可以促进其进一步的应用。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15016/'>《Biotechnology and Applied Biochemistry》</a> <b style="margin: 0 2px;">|</b><span>2018年第3期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huangfu Chaoji&option=202" target="_blank" rel="nofollow">Huangfu Chaoji;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Jinchao&option=202" target="_blank" rel="nofollow">Zhang Jinchao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ma Yuyuan&option=202" target="_blank" rel="nofollow">Ma Yuyuan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jia Junting&option=202" target="_blank" rel="nofollow">Jia Junting;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Jingxuan&option=202" target="_blank" rel="nofollow">Li Jingxuan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lv Maomin&option=202" target="_blank" rel="nofollow">Lv Maomin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ma Xiaowei&option=202" target="_blank" rel="nofollow">Ma Xiaowei;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhao Xiong&option=202" target="_blank" rel="nofollow">Zhao Xiong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Jingang&option=202" target="_blank" rel="nofollow">Zhang Jingang;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Beijing Key Laboratory of Blood Safety and Supply TechnologiesBeijing Institute of Transfusion MedicineBeijing People's Republic of China;</p> <p>Beijing Key Laboratory of Blood Safety and Supply TechnologiesBeijing Institute of Transfusion MedicineBeijing People's Republic of China;</p> <p>Beijing Key Laboratory of Blood Safety and Supply TechnologiesBeijing Institute of Transfusion MedicineBeijing People's Republic of China;</p> <p>Beijing Key Laboratory of Blood Safety and Supply TechnologiesBeijing Institute of Transfusion MedicineBeijing People's Republic of China;</p> <p>Beijing Key Laboratory of Blood Safety and Supply TechnologiesBeijing Institute of Transfusion MedicineBeijing People's Republic of China;</p> <p>Beijing Key Laboratory of Blood Safety and Supply TechnologiesBeijing Institute of Transfusion MedicineBeijing People's Republic of China;</p> <p>Hualan Biological Engineering Inc.Xinxiang People's Republic of China;</p> <p>Beijing Key Laboratory of Blood Safety and Supply TechnologiesBeijing Institute of Transfusion MedicineBeijing People's Republic of China;</p> <p>Beijing Key Laboratory of Blood Safety and Supply TechnologiesBeijing Institute of Transfusion MedicineBeijing People's Republic of China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/177.html" title="生物化学">生物化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=α1‐antitrypsin&option=203" rel="nofollow">α1‐antitrypsin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=blood product&option=203" rel="nofollow">blood product;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chromatography&option=203" rel="nofollow">chromatography;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cohn Fraction IV&option=203" rel="nofollow">Cohn Fraction IV;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=plasma protein&option=203" rel="nofollow">plasma protein;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=viral safety&option=203" rel="nofollow">viral safety;</a> </p> <div class="translation"> 机译：α1-抗抗糖浆;血液产品;色谱法;COHN馏分IV;血浆蛋白质;病毒安全性; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704028288077.html">Large‐scale purification of high purity α1‐antitrypsin from Cohn Fraction IV with virus inactivation by solvent/detergent and dry‐heat treatment</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huangfu Chaoji&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Huangfu Chaoji,</a> <a 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R.,</a> <span>2017</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用热，离子洗涤剂和还原剂灭活西尼罗河病毒的蛋白质组学应用。</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-pathophysiology_thesis/0201223450854.html">乙型肝炎病毒诱导小鼠抗β1-肾上腺素能受体抗体的致心律失常作用机种</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘坤&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘坤</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=廖玉华&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,廖玉华</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王朝晖&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王朝晖</a> <span> <a href="/journal-cn-5784/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国病理生理杂志 </a> </span> <span> . 2005</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_monoclonal-antibody-communication_thesis/0201295528440.html">高效液相色谱纯化抗肾综合征出血热病毒血凝素单克隆抗体及特性鉴定</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=薛小平&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 薛小平</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=商澎&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,商澎</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=汪美先&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,汪美先</a> <span> <a href="/journal-cn-51997/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 单克隆抗体通讯 </a> </span> <span> . 1991</span><span>,第2期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020311309346.html">酵母表达肽抗生素hPAB-β的纯化及其抗单纯疱疹病毒活性分析</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈志瑾&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈志瑾</a> <span> . 2009</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061201107406.html">一种流感病毒血凝素大规模分离纯化方法</a> <b>[P]</b> . <span> 中国专利： CN102190701B </span> <span> . 2013.01.23</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120106401920.html">一种流感病毒血凝素大规模分离纯化方法</a> <b>[P]</b> . <span> 中国专利： CN102190701A </span> <span> . 2011-09-21</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130454189239.html">Process for the extraction and purification of alfa1-antitrypsin (alfa1-pI) from Cohn Fraction IV-1.</a> <b>[P]</b> . <span> 外国专利：  ES2117197T3 </span> <span> . 1998-08-01</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从科恩级分IV-1中提取和纯化α1-抗胰蛋白酶（α1-pI）的方法。 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130453969341.html">Process for the extraction and purification of alfa1-antitrypsin (alfa1-pI) from cohn IV-1 fraction</a> <b>[P]</b> . <span> 外国专利：  EP0717049B1 </span> <span> . 1998-04-01</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从cohn IV-1组分中提取和纯化α1-抗胰蛋白酶（α1-pI）的方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130457111560.html">Process for the extraction and purification of alfa1-antitrypsin (alfa1-pI) from cohn IV-1 fraction</a> <b>[P]</b> . <span> 外国专利：  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