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首页> 外文期刊>Biotechnology and Applied Biochemistry >Improved pulp bleaching potential of Bacillus subtilisBacillus subtilis WB800 through overexpression of three lignolytic enzymes from various bacteria
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Improved pulp bleaching potential of Bacillus subtilisBacillus subtilis WB800 through overexpression of three lignolytic enzymes from various bacteria

机译：改进 bacillus subtilis 芽孢杆菌的髓凋亡潜力通过来自各种细菌的三种钙钼酶的过度表达

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Abstract > A chemical bleaching process of paper pulps gives off excessive amount of chlorinated organic wastes mostly released to environment without exposing complete bioremediaton. Recent alternative and eco‐friendly approaches toward pulp bleaching appear more responsive to environmental awareness. Here we report, direct use of a recombinant Bacillus subtilis bacterium for pulp bleaching, endowed with three ligninolytic enzymes from various bacteria. In addition, efficient bleaching performance from glutathione‐S‐transferase (GST) biocatalyst tested for the first time in pulp bleaching applications was also achieved. Simultaneous and extracellular overproduction of highly active GST, laccase, and lignin peroxidase catalysts were also performed by Bacillus cells. Both enhanced bleaching success and improved delignification rates were identified when enzyme combinations tested on both pine kraft and waste paper pulps, ranging from 69.75% to 79.18% and 60.89% to 74.65%, respectively. Furthermore, when triple enzyme combination applied onto the papers from pine kraft and waste pulps, the best ISO brightness values were identified as 66.45% and 64.67%, respectively. The delignification rates of pulp fibers exposed to various enzymatic bleaching sequences were comparatively examined under SEM. >In conclusion, the current study points out that in near future, a more fined‐tuned engineering of pulp‐colonizing bacteria may become a cost‐effective and environmentally friendly alternative to chemical bleaching. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >化学物质纸浆的漂白过程发出过量的氯化有机废物，主要释放到环境中而不暴露完整的生物ediaton。最近的替代和环保方法对纸浆漂白的途径更加响应于环境意识。在这里，我们报告，直接使用重组的枯草芽孢杆菌枯草芽孢杆菌，用于浆漂白剂，赋予来自各种细菌的三种木质素溶酶。此外，还实现了来自谷胱甘肽-S-转移酶（GST）生物催化剂的高效漂白性能，这是第一次在纸浆漂白剂中测试的生物催化剂。同时和细胞外，通过杆菌（Bacillus）细胞也进行了高活性GST，漆酶和木质素过氧化物酶催化剂。当酶组合在杉木牛皮纸和废纸纸浆上测试的酶组合时，鉴定增强的漂白成功和改善的去脱股税率分别为69.75％至79.18％和60.89％至74.65％。此外，当施加到松果和废纸浆上纸上的三倍酶组合时，最佳的ISO亮度值分别被鉴定为66.45％和64.67％。暴露于各种酶促漂白序列的纸浆纤维的脱磷酸盐率在SEM下进行了相对较好。总之，目前的研究指出，在不久的将来，果实殖民化细菌的更加罚款工程可能成为化学漂白的成本效益和环保的替代品。</ p> < /摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15016/'>《Biotechnology and Applied Biochemistry》</a> <b style="margin: 0 2px;">|</b><span>2018年第4期</span><b style="margin: 0 2px;">|</b><span>共12页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ozer Aysegul&option=202" target="_blank" rel="nofollow">Ozer Aysegul;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Uzuner Ugur&option=202" target="_blank" rel="nofollow">Uzuner Ugur;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guler Halil Ibrahim&option=202" target="_blank" rel="nofollow">Guler Halil Ibrahim;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ay Sal Fulya&option=202" target="_blank" rel="nofollow">Ay Sal Fulya;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Belduz Ali Osman&option=202" target="_blank" rel="nofollow">Belduz Ali Osman;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Deniz Ilhan&option=202" target="_blank" rel="nofollow">Deniz Ilhan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Canakci Sabriye&option=202" target="_blank" rel="nofollow">Canakci Sabriye;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of BiologyKaradeniz Technical UniversityTrabzon Turkey;</p> <p>Department of Molecular Biology and GeneticsKaradeniz Technical UniversityTrabzon Turkey;</p> <p>Department of Molecular Biology and GeneticsKaradeniz Technical UniversityTrabzon Turkey;</p> <p>Department of BiologyKaradeniz Technical UniversityTrabzon Turkey;</p> <p>Department of BiologyKaradeniz Technical UniversityTrabzon Turkey;</p> <p>Department of Forest Industrial EngineeringKaradeniz Technical UniversityTrabzon Turkey;</p> <p>Department of BiologyKaradeniz Technical UniversityTrabzon Turkey;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/177.html" title="生物化学">生物化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=pulp bleaching&option=203" rel="nofollow">pulp bleaching;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=glutathione‐S‐transferase&option=203" rel="nofollow">glutathione‐S‐transferase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=laccase&option=203" rel="nofollow">laccase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lignin peroxidase&option=203" rel="nofollow">lignin peroxidase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=operon cloning&option=203" rel="nofollow">operon cloning;</a> </p> <div class="translation"> 机译：纸浆漂白;谷胱甘肽-S-转移酶;漆酶;木质素过氧化物酶;操纵子克隆; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881059.html">Improved pulp bleaching potential of <i xmlns="http://www.wiley.com/namespaces/wiley">Bacillus subtilisBacillus subtilis WB800 through overexpression of three lignolytic enzymes from various bacteria</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ozer Aysegul&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ozer Aysegul,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Uzuner Ugur&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Uzuner Ugur,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guler Halil Ibrahim&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guler Halil Ibrahim,</a> <a href="/journal-foreign-15016/" target="_blank" rel="nofollow" class="tuijian_authcolor">Biotechnology and Applied Biochemistry .</a> <span>2018</span><span>,第4期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：改进<i xmlns 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