A Preliminary Investigation of Viral Pathogen Causing Foetal Abortion in Donkeys at Ethiopia

摘要

Rabbit kidney (RK13) cells infected with virus isolated from a homogenate made from the placenta of aborted foetal donkeys were stained with Leishman's stain, motphological changes including cytopathic effects (CPE), syncytia, and inclusion bodies were sefenty light microscopy after incubation for 24hrs-96hrs at 37°C. After 48hrs of incubation, about 60% of cells were infected. Another Set of RK 13 cells infected with either native virus or both ether treated virus and native virus in the presence of acyclovir was stained with Giemsa, morphology changes were observed in the native virus infected cell while little or no change was seen in infected cells in the presence of acyclovir and ether treated virus respectively. Virus infected RK13 cells were stained with acridine orange, intracellular fluorescent green colour was seen by fluorescence microscopy in the cell nucleus. The clinical history and CPE of the virus in RK13 cell are similar to Equine Herpes virus.