Extraction, purification, and characterisation of the flavonoids from Opuntia milpa alta skin.

摘要

Flavonoids contained in Opuntia ficus-indica (var. milpa alta) skin were extracted, purified and identified. Based on single factor experimental results, the extraction parameters were optimised using an orthogonal design and variance analysis. Max. yield of flavonoids (5.55 mg/g dry wt.) was obtained using 80% ethanol (v/v), an extraction temp. of 90 degrees C and a solvent to raw material ratio of 25:1. After purification with AB-8 resin, the main components of the extract were characterised as isorhamnetin 3-O-(2, 6-dirhamnosyl)glucoside and isorhamnetin 3-O-D-rutinoside by LC-MS and UV-visible spectrometry.