On-chip pulsed electromembrane extraction as a new concept for analysis of biological fluids in a small device

摘要

Highlights?An on-chip pulsed electromembrane extraction (On-chip-PEME) technique was developed.?Method was applied for extraction of codeine, naloxone and naltrexone from biological fluids.?Separation and analysis of the extracted analytes were performed by HPLC-UV.?Effective parameters of the method were optimized using one-variable-at-a-time method.?Under optimized conditions, model analytes were effectively extracted from different matrices.AbstractIn the present work, an on-chip pulsed electromembrane extraction technique followed by HPLC-UV was developed for the analysis of codeine, naloxone and naltrexone as model analytes in biological fluids. The chip consisted of two channels for the introduction of the donor and acceptor phases. The channels were carved in two poly (methyl methacrylate